公开(公告)号：US07687239B2

公开(公告)日：2010-03-30

申请号：US10888828

申请日：2004-07-08

Applicant: David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/53

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

Abstract translation: 本发明涉及将微生物移动到表面，其中它们在存在和不存在抗微生物剂的情况下生长，并且通过在两个条件下随时间监测微生物的生长，可以确定它们对抗微生物剂的敏感性。 微生物可通过电泳，离心或过滤移至表面。 当运动涉及电泳时，氧化和还原剂的存在降低了可以产生电泳力的电压，并允许可以检测目标的更宽范围的方法。 监测可以包括光学检测，并且最方便地包括检测单个微生物。 微生物可以被染色以便提供关于它们对抗微生物剂的反应的信息。