公开(公告)号：US20210255174A1

公开(公告)日：2021-08-19

申请号：US17020658

申请日：2020-09-14

申请人： CITY OF HOPE

发明人： Markus KALKUM ,  Karine BAGRAMYAN

IPC分类号： G01N33/53 ,  C07K14/705 ,  C07K7/08 ,  G01N33/542 ,  G01N33/569 ,  G01N33/573

摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

公开(公告)号：US11077473B2

公开(公告)日：2021-08-03

申请号：US16253945

申请日：2019-01-22

申请人： City of Hope

发明人： Gagandeep Singh

IPC分类号： A46B3/18 ,  B08B9/043 ,  A61B17/00 ,  B08B1/00 ,  B08B1/04

摘要： A cleaner device is adapted to clean a blood vessel sealer device. The cleaner device includes a series of brushes that can move relative to blood vessel sealer device to scrub and clean the blood vessel sealer device when coupled to the cleaner device. The brushes can be made of any material that is adapted to clean the blood vessel sealer device. The cleaner device can also be motorized to facilitate cleaning of the blood vessel sealer device.

公开(公告)号：US11058700B2

公开(公告)日：2021-07-13

申请号：US15338158

申请日：2016-10-28

申请人： City of Hope

发明人： Peter P. Lee ,  Dobrin Draganov

IPC分类号： A61K31/7048 ,  A61K35/768 ,  A61K31/704 ,  A61K31/337

摘要： Described herein, inter alia, are macrocyclic lactones such as ivermectin and methods for their use in treating, for example, hyperproliferative disorders such as cancer.

公开(公告)号：US20210145973A1

公开(公告)日：2021-05-20

申请号：US16630343

申请日：2018-07-13

申请人： CITY OF HOPE

发明人： Andreas HERRMANN ,  Hua YU ,  Toshikage NAGAO

IPC分类号： A61K47/54 ,  A61K38/17 ,  A61P35/00 ,  A61P29/00 ,  A61P31/18

摘要： Provided herein are, inter alia, nucleic acid-peptide conjugates including a non-cell penetrating protein attached at its C-terminus to a phosphorothioate nucleic acid. Attachment of the phosphorothioate nucleic acid to the non-cell penetrating protein conveys stability to and allows for efficient intracellular delivery of the non-cell penetrating peptide. The nucleic acid-peptide conjugates provided herein including embodiments thereof are useful, inter alia, for the treatment of cancer, inflammatory disease, pain, and viral infection.

公开(公告)号：US20210139476A1

公开(公告)日：2021-05-13

申请号：US16623730

申请日：2018-06-21

申请人： CITY OF HOPE ,  NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS

发明人： Sangkil Nam ,  David Horne ,  Ravi Salgia ,  Alexios-Leandros Skaltsounis ,  Nikolaos Lougiakis ,  Nicole Pouli ,  Panagiotis Marakos

IPC分类号： C07D471/04

摘要： Disclosed herein, inter alia, are pyridopyrazines and methods of using the same.

公开(公告)号：US20210130789A1

公开(公告)日：2021-05-06

申请号：US17082642

申请日：2020-10-28

申请人： CITY OF HOPE

发明人： Ismail H. AL-ABDULLAH ,  Meirigeng QI ,  Hsun Terasa KU ,  Bashar KHIATAH

IPC分类号： C12N5/0775 ,  C12N5/071

摘要： Disclosed herein are methods for expanding and potentiating intra-pancreatic tissue-derived mesenchymal stromal/stem cells in the presence of TNF-α, DMOG, or both, to obtain IPTD-MSCs having enhanced pro-angiogenic and/or anti-inflammatory properties. Also disclosed are therapeutic applications of these IPTD-MSCs.

公开(公告)号：US10967070B2

公开(公告)日：2021-04-06

申请号：US15056777

申请日：2016-02-29

申请人： City of Hope

发明人： Andreas Herrmann ,  Hua Yu ,  Piotr Marek Swiderski ,  John Ernest Shively ,  Lin Li

IPC分类号： A61K47/50 ,  C07K16/08 ,  C07K16/40 ,  C07K16/18 ,  A61K39/44 ,  A61K47/68 ,  A61K47/54 ,  A61K39/00 ,  A61K38/00 ,  C12N15/11

摘要： Provided herein are cell penetrating conjugates. The conjugates include a non-cell penetrating protein attached to a phosphorothioate nucleic acid or phosphorothioate polymer backbone, wherein the phosphorothioate nucleic acid or phosphorothioate polymer backbone enhances intracellular delivery of the non-cell penetrating protein. Also provided are compositions and kits comprising the cojugates.

公开(公告)号：US10961538B2

公开(公告)日：2021-03-30

申请号：US16694462

申请日：2019-11-25

申请人： CITY OF HOPE

发明人： Don J. Diamond ,  Edwin Manuel

IPC分类号： A01N63/00 ,  C12N15/113 ,  A61K39/00 ,  A61K39/39 ,  C12N1/20

摘要： In one embodiment, a single modality cancer immunotherapy regimen that includes a therapeutic composition is provided. Such a therapeutic composition may include a Salmonella strain comprising a plasmid that expresses an shRNA molecule that suppresses the expression of an immunosuppressive target and suppresses tumor growth. In some aspects, the Salmonella strain is an attenuated Salmonella typhimurium strain. In other aspects, the immunosuppressive target is STAT3, IDO1, IDO2, Arginase 1, iNOS, CTLA-4, TGF-β, IL-10, pGE2 or VEGF. In one embodiment, the immunosuppressive target is IDO1 or Arg1 and the shRNA molecule is any one of SEQ ID NO:5-14.

公开(公告)号：US20210077535A1

公开(公告)日：2021-03-18

申请号：US16999459

申请日：2020-08-21

申请人： City of Hope

发明人： Marcin Tomasz Kortylewski ,  Karen Aboody ,  Alexandra Jacqueline Annala ,  Mohamed Hammad

IPC分类号： A61K35/30 ,  C12N5/0797 ,  C12N15/113 ,  A61P35/00

摘要： The disclosure provides, inter alia, neural stem cells comprising exogenous nucleotides, vesicles comprising exogenous nucleotides, and methods for treating diseases and disorders, such as cancer, neurodegenerative diseases, inflammatory diseases, and viral diseases, using the neural stem cells, vesicles, and pharmaceutical compositions comprising them.

公开(公告)号：US10946061B2

公开(公告)日：2021-03-16

申请号：US16843718

申请日：2020-04-08

申请人： City of Hope

发明人： Carlotta A. Glackin

IPC分类号： A61K38/10 ,  A61K47/64 ,  A61K45/06 ,  C07K14/47 ,  A61K38/17 ,  A61K31/337 ,  A61K31/555 ,  A61K31/4745 ,  A61K33/24

摘要： The disclosure provides, inter alia, compositions comprising TWIST peptide inhibitors and optionally one or more anti-cancer agents, and methods of using the compositions for the treatment of cancer.