公开(公告)号：US20220267928A1

公开(公告)日：2022-08-25

申请号：US17703849

申请日：2022-03-24

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C30B29/40 ,  H01L33/00 ,  H01L33/32 ,  C30B29/38 ,  H01L21/205

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20220259620A1

公开(公告)日：2022-08-18

申请号：US17703861

申请日：2022-03-24

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/686 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US11293034B2

公开(公告)日：2022-04-05

申请号：US17326791

申请日：2021-05-21

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C12N15/63 ,  C12Q1/686 ,  C12N15/113 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US11008590B2

公开(公告)日：2021-05-18

申请号：US17102059

申请日：2020-11-23

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  A61K38/46 ,  C12N15/90 ,  C12Q1/686 ,  C12N15/70 ,  C12N15/74 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10982230B2

公开(公告)日：2021-04-20

申请号：US16935016

申请日：2020-07-21

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/686 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together, with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20210062225A1

公开(公告)日：2021-03-04

申请号：US17084020

申请日：2020-10-29

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelie Charpentier

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/686 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20210062224A1

公开(公告)日：2021-03-04

申请号：US17084014

申请日：2020-10-29

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/686 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10900054B2

公开(公告)日：2021-01-26

申请号：US16935007

申请日：2020-07-21

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  A01H6/46 ,  A61K48/00 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01K67/027 ,  C12N15/90 ,  C12Q1/686

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10774344B1

公开(公告)日：2020-09-15

申请号：US16892631

申请日：2020-06-04

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/63 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  C12N15/11 ,  C12N15/90 ,  C12Q1/686 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10695474B2

公开(公告)日：2020-06-30

申请号：US15513519

申请日：2015-09-22

申请人： St Vincent's Hospital Sydney Limited ,  Medical University of Vienna

发明人： Marcus Granegger ,  Francesco Moscato ,  Heinrich Schima ,  Christopher Simon Hayward

IPC分类号： A61M1/12 ,  A61M1/10

摘要： Apparatus for determining opening of an aortic valve of a biological subject, the apparatus including an electronic processing device that determines a pump speed of a ventricular assist device that is assisting cardiac function of the biological subject, analyses the pump speed to determine a pump speed indicator at least partially indicative of changes in pump speed and uses the pump speed indicator to determine an opening indicator indicative of opening of the aortic valve.