公开(公告)号：US20070083952A1

公开(公告)日：2007-04-12

申请号：US11545076

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070083947A1

公开(公告)日：2007-04-12

申请号：US11544928

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US07151204B2

公开(公告)日：2006-12-19

申请号：US09757089

申请日：2001-01-09

申请人： Nancy Houmard ,  Lucille B. Laccetti ,  Albert P. Kausch ,  Emil M. Orozco, Jr.

发明人： Nancy Houmard ,  Lucille B. Laccetti ,  Albert P. Kausch ,  Emil M. Orozco, Jr.

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8223 ,  C12N9/88

摘要： The current invention provides the promoter of the Zea mays nuclear gene encoding chloroplast-localized fructose-1,6-bisphosphate (F16BP) aldolase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the chloroplastic F16BP aldolase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

摘要翻译： 本发明提供了编码叶绿体定位的果糖-1,6-二磷酸（F16BP）醛缩酶的玉米核基因的启动子。 描述了包含该序列的组合物，以及用这种组合物转化的植物。 还提供了在植物中表达转基因的方法，其中包括使用这些序列。 本发明的方法包括通过遗传转化直接产生具有叶绿体F16BP醛缩酶启动子的转基因植物，以及通过植物育种方法。 本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。