公开(公告)号：US07754433B2

公开(公告)日：2010-07-13

申请号：US10309421

申请日：2002-12-02

申请人： John Babcook ,  Xiao-Chi Jia ,  Jaspal Singh Kang ,  Li Zhang

发明人： John Babcook ,  Xiao-Chi Jia ,  Jaspal Singh Kang ,  Li Zhang

IPC分类号： G01N33/53

CPC分类号： G01N33/6854 ,  G01N33/6857

摘要： Methods are disclosed for determining the relative binding affinities of molecules for their binding partner. One type of molecule is an antibody, which has an antigen binding partner. Antibodies are tested for binding against limited dilutions of antigen. Antibodies that bind to the most dilute antigen concentrations are determined to have a higher relative binding affinity for the antigen than antibodies that only bind to more concentrated antigen preparations.

摘要翻译： 公开了用于确定其结合配偶体的分子的相对结合亲和力的方法。 一种分子是具有抗原结合配偶体的抗体。 测试抗体与抗原的有限稀释度结合。 确定与最稀释的抗原浓度结合的抗体比仅与结合更浓缩的抗原制剂的抗体相比具有比抗原更高的相对结合亲和力。