公开(公告)号：US4814057A

公开(公告)日：1989-03-21

申请号：US143546

申请日：1988-01-13

申请人： Hideyuki Nishizawa

发明人： Hideyuki Nishizawa

IPC分类号： B01D57/02 ,  B01D63/00 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  G01N27/44708

摘要： An open top electrode tank including two electrode chambers respectively containing an electrolyte and an electrode, and an open top cooling tank containing cooling fluid are superposed one upon the other. A separating zone is attached to the bottom of the cooling tank, and bent portions of a pair of bridging filter papers are attached to predetermined positions of a separating zone. Permanent magnets are disposed on both sides of each filter paper and on the bottom of the cooling tank for attracting each other.

摘要翻译： 包括分别含有电解质和电极的两个电极室和容纳冷却流体的敞开顶部冷却槽的开放顶部电极槽彼此重叠。 分离区连接到冷却箱的底部，一对桥接滤纸的弯曲部分附接到分离区的预定位置。 永久磁铁设置在每个滤纸的两侧和冷却箱的底部，用于彼此吸引。

公开(公告)号：US4624768A

公开(公告)日：1986-11-25

申请号：US695006

申请日：1985-01-25

申请人： Motoko Yoshida ,  Michio Itoh ,  Kunihiro Maeda

发明人： Motoko Yoshida ,  Michio Itoh ,  Kunihiro Maeda

IPC分类号： G01N27/26 ,  B01D57/02 ,  G01N27/447 ,  G01N27/28

CPC分类号： G01N27/44708

摘要： A horizontal electrophoresis apparatus characterized by a cooling plate which consists of a sintered SiC with high thermal conductivity and good insulating properties, or sintered material composed mainly of SiC, and a Peltier element, as the heat conducting material to cool the supporting matrix or electrolyte.

摘要翻译： 一种水平电泳装置，其特征在于具有高导热性和良好绝缘性的烧结SiC，或主要由SiC组成的烧结材料和珀耳帖元件组成的冷却板，作为冷却支撑基质或电解质的导热材料。

公开(公告)号：US4612106A

公开(公告)日：1986-09-16

申请号：US685798

申请日：1984-12-24

申请人： Heiner M. Kromer ,  Daniel November

发明人： Heiner M. Kromer ,  Daniel November

IPC分类号： G01N27/447 ,  G01N27/28

CPC分类号： G01N27/44708

摘要： Disclosed herein is a gel slab electrophoresis device having novel temperature and heat control capability. The electrophoresis device includes a sample unit having a pair of transversely-spaced first and second plates containing a gel cast, these plates having open top and bottom slots spaced therebetween. An integral part of the apparatus is a heat sink which is in thermal communication with the sample unit, the heat sink constituting a pneumatic circuit for both heat distribution and removal to and from the gel cast. There is also provided upper and lower solution chambers, both filled with conductive solutions, and both in fluid communication with the respective top and bottom portions of the gel. The apparatus also includes an arrangement for applying an electrical potential between the upper and lower solution chambers and, thereby, across the entire vertical length of the gel. Additionally provided is a refrigeration sub-system, the output of which is heat exchanged into the pneumatic circuit.

摘要翻译： 本文公开了具有新的温度和热控制能力的凝胶板电泳装置。 电泳装置包括具有一对横向隔开的第一和第二板的样品单元，该第一和第二板含有凝胶铸件，这些板具有间隔开的顶部和底部开口。 设备的一个组成部分是与样品单元热连通的散热器，散热器构成用于从凝胶铸件中分离和去除凝胶铸件的气动回路。 还提供了上部和下部溶液室，两者都填充有导电溶液，并且两者都与凝胶的相应顶部和底部流体连通。 该装置还包括用于在上下溶液室之间施加电势，从而在凝胶的整个垂直长度上施加电势的装置。 另外提供了一种制冷子系统，其输出被热交换到气动回路中。

公开(公告)号：US3932263A

公开(公告)日：1976-01-13

申请号：US520286

申请日：1974-11-04

申请人： Paul E. Brefka

发明人： Paul E. Brefka

IPC分类号： G01N27/26 ,  G01N27/447 ,  B01K5/00

CPC分类号： G01N27/44708

摘要： Apparatus for holding an electrophoresis slide of agarose gel against the concave surface of a slide mounting block is described. The holding means permits the slide to be inserted readily and holds the slide in compression in intimate contact with the concave surface.

摘要翻译： 描述了用于将滑动安装块的凹面保持琼脂糖凝胶电泳载玻片的装置。 保持装置允许滑动件容易地插入并且将滑块保持在与凹面紧密接触的压缩状态。

公开(公告)号：US20180095055A1

公开(公告)日：2018-04-05

申请号：US15554582

申请日：2016-03-07

申请人： 4D Lifetec AG

发明人： Oliver Schicht

IPC分类号： G01N27/447 ,  B01L9/00

CPC分类号： G01N27/44782 ,  B01L9/54 ,  B01L9/56 ,  B01L2300/0829 ,  G01N27/44704 ,  G01N27/44708

摘要： A gel electrophoresis apparatus for single cell gel electrophoresis, including a chamber for receiving a gel electrophoresis buffer, a functional cover for closing the chamber, at least one pair of electrodes for generating a homogeneous electric field in the chamber and at least one retaining element for receiving and positioning at least one support plate. The at least one retaining element positions the at least one support plate in the homogeneous electric field generated by the at least one pair of electrodes.

公开(公告)号：US20170131234A1

公开(公告)日：2017-05-11

申请号：US15017540

申请日：2016-02-05

申请人： Woodham Biotechnology Holdings, LLC

发明人： Andrew Woodham

IPC分类号： G01N27/447 ,  G01N33/531

CPC分类号： G01N27/44739 ,  G01N27/44708 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/4473

摘要： A precast gel/membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive plastic/polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive plastics/polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electrical current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive plastics/polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel/membrane combination unit. The apparatus allows the user to visually see and perform the steps of protein separation and protein transfer to a transfer membrane without transferring the gel or apparatus after the electrophoresis separation phase.

公开(公告)号：US20170102359A1

公开(公告)日：2017-04-13

申请号：US15128032

申请日：2014-05-08

申请人： Chao YAN

发明人： Chao YAN ,  Dong YAO ,  Lin ZHANG ,  Jing LI

IPC分类号： G01N27/447

CPC分类号： G01N27/44743 ,  G01N27/44708 ,  G01N27/44756 ,  G01N27/44791

摘要： A fully automated high-precision capillary electrophoresis instrument, comprising an electrophoresis system, a sample injection flow path, and an automatic sampling flow path; the sampling flow path comprising a shunt waste bottle, which is connected to a four-way connector, a four-way sample injection valve and a buffer syringe pump; the automatic sampling flow path comprises a sampling needle, a sample tray, a cleaning tank, reagent bottles, a buffer tube, a six-channel liquid dispenser, and a syringe pump. The described capillary electrophoresis instrument has a fast sample injection speed, high accuracy, good reproducibility, and can be widely used in automated analysis of different substances by capillary electrophoresis.

公开(公告)号：US20120241322A1

公开(公告)日：2012-09-27

申请号：US13427037

申请日：2012-03-22

申请人： Yasunori Shiraki ,  Daisuke Matsumoto ,  Yusuke Nakayama ,  Genki Adachi

发明人： Yasunori Shiraki ,  Daisuke Matsumoto ,  Yusuke Nakayama ,  Genki Adachi

IPC分类号： G01N27/26 ,  G01N27/453

CPC分类号： G01N27/44708 ,  B01D57/02 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/44791

摘要： An analysis apparatus is an apparatus that performs electrophoresis using a microchip provided with a channel. The analysis apparatus includes a cooling unit (an electron cooling element and a driving circuit) that cools the microchip, a voltage application unit (electrodes and a power supply circuit) that applies voltage to a buffer solution filled in the channel of the microchip, an optical analysis unit (a light source, a light receiving element, and an analysis unit) that conducts, through the microchip, optical analysis of a sample introduced in the channel, and a control unit that controls the cooling unit, the voltage application unit, and the optical analysis unit. The control unit causes the cooling unit to start cooling the microchip, and after the microchip has been cooled, causes the voltage application unit and the optical analysis unit to operate.

摘要翻译： 分析装置是使用设置有通道的微芯片进行电泳的装置。 分析装置包括冷却微芯片的冷却单元（电子冷却元件和驱动电路），向填充在微芯片的通道中的缓冲溶液施加电压的电压施加单元（电极和电源电路）， 光学分析单元（光源，光接收元件和分析单元），其通过微芯片对通道中引入的样品进行光学分析;以及控制单元，其控制冷却单元，电压施加单元， 和光学分析单元。 控制单元使冷却单元开始冷却微芯片，并且在微芯片冷却之后，使电压施加单元和光学分析单元工作。

公开(公告)号：US07459068B2

公开(公告)日：2008-12-02

申请号：US10309729

申请日：2002-12-04

申请人： Eric S. Nordman ,  Miho Ozawa ,  Masaya Kojima ,  Ryoji Inaba ,  Yoshitaka Kodama ,  Motohiro Yamazaki

发明人： Eric S. Nordman ,  Miho Ozawa ,  Masaya Kojima ,  Ryoji Inaba ,  Yoshitaka Kodama ,  Motohiro Yamazaki

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44704 ,  G01N27/44708 ,  G01N27/44782

摘要： A multi-capillary electrophoresis apparatus is provided for reducing errors during analysis caused by fluctuations in electrophoresis time among plural capillaries of the apparatus. The multi-capillary electrophoresis apparatus can contain a multi-capillary array that has a separation medium filled therein for isolating a sample. A detector component can be provided at a position remote from a sample injecting end of the array for acquiring information from the sample. A buffer container can be provided for holding a buffer solution into which the sample injecting end can be immersed. One or more temperature controlling component devices can be arranged for controlling a temperature of the buffer solution, of the detector component, or of both the buffer solution and the detector component.

摘要翻译： 提供了一种多毛细管电泳装置，用于减少由于装置的多个毛细管之间的电泳时间波动引起的分析过程中的误差。 多毛细管电泳装置可以包含多个毛细管阵列，其具有填充在其中的分离介质，用于分离样品。 可以在远离阵列的样品注入端的位置处提供检测器部件，以从样品获取信息。 可以提供缓冲容器用于保持可以浸入样品注入端的缓冲溶液。 可以布置一个或多个温度控制部件装置，用于控制缓冲溶液，检测器部件或缓冲溶液和检测器部件两者的温度。

公开(公告)号：US07169276B2

公开(公告)日：2007-01-30

申请号：US10075404

申请日：2002-02-13

申请人： Ben F. Johnson ,  Eric S. Nordman

发明人： Ben F. Johnson ,  Eric S. Nordman

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  G01N27/44708

摘要： Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

摘要翻译： 描述了在毛细管电泳过程中与运行场的建立相关的峰拓宽减少的方法以及用于实施这些方法的装置。 这样的方法包括定义在初始电场斜坡期间要使用的最大斜坡速率，定义运行场建立的最小周期和/或在初始电场斜坡期间将分离介质的温度保持在特定范围内 。 此外，公开了用于确定有效减少峰值变宽的斜坡率的方法。