Gel Electrophoresis and Transfer Combination using Conductive Polymers and Method of Use
摘要:
A precast gel/membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive plastic/polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive plastics/polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electrical current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive plastics/polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel/membrane combination unit. The apparatus allows the user to visually see and perform the steps of protein separation and protein transfer to a transfer membrane without transferring the gel or apparatus after the electrophoresis separation phase.
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