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公开(公告)号：US20170356876A1
公开(公告)日：2017-12-14
申请号：US15688738
申请日：2017-08-28
发明人： Andrew Woodham
IPC分类号： G01N27/447
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44721
摘要： A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membrane combination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
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2.发明申请公开(公告)号：US20170131234A1
公开(公告)日：2017-05-11
申请号：US15017540
申请日：2016-02-05
发明人： Andrew Woodham
IPC分类号： G01N27/447 , G01N33/531
CPC分类号： G01N27/44739 , G01N27/44708 , G01N27/44713 , G01N27/44721 , G01N27/4473
摘要： A precast gel/membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive plastic/polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive plastics/polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electrical current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive plastics/polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel/membrane combination unit. The apparatus allows the user to visually see and perform the steps of protein separation and protein transfer to a transfer membrane without transferring the gel or apparatus after the electrophoresis separation phase.
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公开(公告)号：US10203304B2
公开(公告)日：2019-02-12
申请号：US15639317
申请日：2017-06-30
发明人： Andrew Woodham
IPC分类号： G01N27/447
摘要： A precast gel and blotting membrane combination unit and method of use. The device includes two plates, each plate having a conductive opaque region with conductive polymers and a transparent region having static-dissipative polymers. Between the plates are a gel matrix and blotting membrane. The device is placed in a tank capable of both performing the electrophoresis phase and transfer phase of a western blot. During the electrophoresis phase, current flows from a pair of electrophoresis electrodes to separate proteins by size. The user can visualize the extent of protein separation by observing a tracking dye through the transparent region. After the electrophoresis phase, voltage is switched to a pair of transfer phase electrodes. The device allows current to flow through the conductive opaque regions of the plates to transfer separated proteins to a blotting membrane directly after electrophoresis without having to remove or reorient the device in the tank.
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公开(公告)号：US09753008B2
公开(公告)日：2017-09-05
申请号：US15348803
申请日：2016-11-10
发明人： Andrew Woodham
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44721
摘要： A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membrane combination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
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公开(公告)号：US09983168B2
公开(公告)日：2018-05-29
申请号：US15688738
申请日：2017-08-28
发明人： Andrew Woodham
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44721
摘要： A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membrane combination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
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公开(公告)号：US09702851B1
公开(公告)日：2017-07-11
申请号：US15186110
申请日：2016-06-17
发明人： Andrew Woodham
IPC分类号： G01N27/453 , G01N27/447
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44726 , G01N27/44747 , G01N27/44778
摘要： A precast gel and blotting membrane combination unit and method of use. The device includes two plates, each plate having a conductive opaque region with conductive polymers and a transparent region having static-dissipative polymers. Between the plates are a gel matrix and blotting membrane. The device is placed in a tank capable of both performing the electrophoresis phase and transfer phase of a western blot. During the electrophoresis phase, current flows from a pair of electrophoresis electrodes to separate proteins by size. The user can visualize the extent of protein separation by observing a tracking dye through the transparent region. After the electrophoresis phase, voltage is switched to a pair of transfer phase electrodes. The device allows current to flow through the conductive opaque regions of the plates to transfer separated proteins to a blotting membrane directly after electrophoresis without having to remove or reorient the device in the tank.
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7.发明申请公开(公告)号：US20170363574A1
公开(公告)日：2017-12-21
申请号：US15639317
申请日：2017-06-30
发明人： Andrew Woodham
IPC分类号： G01N27/447
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44726 , G01N27/44747 , G01N27/44778
摘要： A precast gel and blotting membrane combination unit and method of use. The device includes two plates, each plate having a conductive opaque region with conductive polymers and a transparent region having static-dissipative polymers. Between the plates are a gel matrix and blotting membrane. The device is placed in a tank capable of both performing the electrophoresis phase and transfer phase of a western blot. During the electrophoresis phase, current flows from a pair of electrophoresis electrodes to separate proteins by size. The user can visualize the extent of protein separation by observing a tracking dye through the transparent region. After the electrophoresis phase, voltage is switched to a pair of transfer phase electrodes. The device allows current to flow through the conductive opaque regions of the plates to transfer separated proteins to a blotting membrane directly after electrophoresis without having to remove or reorient the device in the tank.
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公开(公告)号：US20170131235A1
公开(公告)日：2017-05-11
申请号：US15348803
申请日：2016-11-10
发明人： Andrew Woodham
IPC分类号： G01N27/447
CPC分类号： G01N27/44739 , G01N27/44713 , G01N27/44721
摘要： A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membrane combination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
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