公开(公告)号：US20080226669A1

公开(公告)日：2008-09-18

申请号：US11617414

申请日：2006-12-28

Applicant: Michael Roof ,  Phillip Hayes ,  Marc Eichmeyer ,  Greg Nitzel

Inventor： Michael Roof ,  Phillip Hayes ,  Marc Eichmeyer ,  Greg Nitzel

IPC: A61K39/295 ,  A61K39/12 ,  A61P31/12

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. Also provided is recombinant PCV2 ORF2 protein, and immunogenic compositions comprising PCV2 ORF2 protein. Moreover, multivalent combination vaccines are provided which include an immunological agent effective for reducing the incidence of or lessening the severity of PCV2 infection, preferably PCV2 ORF2 protein, or an immunogenic composition comprising PCV2 ORF2 protein, and at least one immunogenic active component of another disease-causing organism in swine,

Abstract translation: 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 还提供重组PCV2 ORF2蛋白和包含PCV2 ORF2蛋白的免疫原性组合物。 此外，提供了多价组合疫苗，其包括有效降低PCV2感染的严重性（优选PCV2 ORF2蛋白）或包含PCV2 ORF2蛋白的免疫原性组合物的发生率或降低的免疫学试剂，以及至少一种其它疾病的免疫原性活性成分 在猪中有机体，

公开(公告)号：US07910306B2

公开(公告)日：2011-03-22

申请号：US12138020

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: C12Q1/68

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：US07829101B2

公开(公告)日：2010-11-09

申请号：US12137960

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K39/12

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

Abstract translation: 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，导致病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取程序。

公开(公告)号：US20090016992A1

公开(公告)日：2009-01-15

申请号：US12137960

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K35/76 ,  A61K38/00 ,  A61P31/00

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：US20130101621A1

公开(公告)日：2013-04-25

申请号：US13728228

申请日：2012-12-27

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K39/12

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：US07833707B2

公开(公告)日：2010-11-16

申请号：US11034797

申请日：2005-01-13

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: C12Q1/68

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from PCV2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

Abstract translation: 提供了一种用于从PCV2回收由开放阅读框2表达的蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，使病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。

公开(公告)号：US20090022750A1

公开(公告)日：2009-01-22

申请号：US12137938

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K39/12 ,  A61P37/04

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：US07700285B1

公开(公告)日：2010-04-20

申请号：US11319975

申请日：2005-12-29

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: C12Q1/68

CPC classification number: A61K39/12 ,  A61K2039/5258 ,  A61K2039/552 ,  A61K2039/55511 ,  A61K2039/55566 ,  C07K14/005 ,  C12N2750/10022 ,  C12N2750/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

Abstract translation: 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，使病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。

公开(公告)号：US20090022752A1

公开(公告)日：2009-01-22

申请号：US12138020

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K39/12 ,  A61P37/04 ,  A61P31/12

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：US20090022751A1

公开(公告)日：2009-01-22

申请号：US12137950

申请日：2008-06-12

Applicant: Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

Inventor： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC: A61K39/12 ,  A61P37/04

CPC classification number: A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

Abstract: An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.