公开(公告)号：US4499065A

公开(公告)日：1985-02-12

申请号：US489898

申请日：1983-04-29

Applicant: Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

Inventor： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

IPC: A61K38/00 ,  A61K39/35 ,  A61K49/00 ,  C12N9/50 ,  C12N9/99

CPC classification number: A61K49/0006 ,  A61K39/35 ,  C12N9/50 ,  C12N9/99 ,  A61K38/00

Abstract: A chymopapain derivative having the enzymatic activity reduced by at least 95% while retaining the original allergenic activity of at least 90% is suitable for skin testing to detect allergic hypersensitivity to chymopapain and for treating patients exhibiting allergic hypersensitivity to chymopapain. The enzymatic activity can be blocked by reacting thiol groups of the enzyme with and iodoacetic acid, bromoacetic acid, or a salt, ester or amide derivative thereof, under conditions which block the undesirable enzymatic activity while retaining the desired allergenic activity.

Abstract translation: 具有降低至少95％的酶活性并且保持至少90％的原始致敏活性的木瓜凝乳蛋白酶衍生物适用于皮肤测试以检测对木瓜凝乳蛋白酶的过敏性超敏反应，并且用于治疗对木瓜凝乳蛋白酶表现出过敏性过敏的患者。 通过使酶的硫醇基与碘乙酸，溴乙酸或其盐，酯或酰胺衍生物在阻碍不期望的酶活性同时保留所需的变应原活性的条件下，可以阻止酶活性。