公开(公告)号：US20140203818A1

公开(公告)日：2014-07-24

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

CPC classification number: G01N27/02 ,  G01N33/4836

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

Abstract translation: 用于监测微电极阵列上的可兴奋细胞（例如心肌细胞）的装置，系统和方法，其耦合可刺激细胞的电刺激以诱导或调节心肌细胞搏动，以及同时测量阻抗和细胞外记录以评估心肌细胞跳动，活力， 响应于多种处理的形态或电物理性质。

公开(公告)号：US20200292454A1

公开(公告)日：2020-09-17

申请号：US16833235

申请日：2020-03-27

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N15/14

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US20200158670A1

公开(公告)日：2020-05-21

申请号：US16721742

申请日：2019-12-19

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02 ,  G01N33/483

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

公开(公告)号：US20180246079A1

公开(公告)日：2018-08-30

申请号：US15523649

申请日：2016-11-21

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A Abassi ,  Nan Li ,  Lincoln Muir ,  Xiao Xu

IPC: G01N33/50 ,  C12N13/00 ,  C12M1/34 ,  G01N33/487

CPC classification number: G01N33/5011 ,  C12M1/3407 ,  C12N13/00 ,  G01N33/4836 ,  G01N33/48707 ,  G01N33/502 ,  G01N33/5047 ,  G01N2800/52 ,  G01N2800/7028

Abstract: Methods of assessing cytolysis of cancer cells, including providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, wherein the device comprises a well for receiving cells and an electrode array at a base of the well; adding target cells characterized as cancer cells to the well; adding effector cells to the well to form a test well, wherein the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance based parameter over time.