公开(公告)号：US20150148243A1

公开(公告)日：2015-05-28

申请号：US14323812

申请日：2014-07-03

Applicant: Samsung Electronics Co., Ltd.

Inventor： Hun-joo LEE ,  Yeon-jeong Kim ,  Dong-hyun Park ,  Jong-myeon Park

IPC: G01N1/30

CPC classification number: G01N1/30 ,  G01N2001/305

Abstract: A photocleavable polymer comprising a photocleavable linker and a polymer having a functional group that binds to a protein, lipid, sugar of a cell, or any combination thereof, as well as a method of selectively lysing cells by incubating a sample comprising two or more cells with the photocleavable polymer to reversibly fix the cells; selectively irradiating the fixed cells or a target or non-target cell among the fixed cells to cleave the photocleavable polymer; and adding a cell lysis solution to selectively lyse the irradiated cells.

Abstract translation: 包含可光切割接头和具有结合蛋白质，脂质，细胞糖或其任何组合的官能团的聚合物的可光致透照聚合物，以及通过将包含两个或多个细胞的样品 用可透光聚合物可逆地固定细胞; 选择性地照射固定细胞中的固定细胞或靶或非靶细胞以切割可光裂解的聚合物; 并加入细胞裂解溶液以选择性地裂解照射的细胞。