公开(公告)号：US10438047B2

公开(公告)日：2019-10-08

申请号：US15725413

申请日：2017-10-05

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu Iga ,  Shinichi Takimoto ,  Yohei Tanikawa ,  Yoshinobu Akahori

IPC: G06K9/00 ,  C12M1/00 ,  C12Q1/06 ,  G06T7/13 ,  G06T7/00 ,  C12M1/34 ,  G01N15/06 ,  G01N15/14

Abstract: For the purpose of counting the number of cells within a culture container readily and accurately, a cell analysis device includes a cell-image acquiring unit that acquires an image of cells within a culture container in which the cells are cultured, a usability determining unit that determines whether or not the image acquired by the cell-image acquiring unit is usable, a number-of-cells counting unit that counts the number of cells within the image determined as being usable by the usability determining unit, and a number-of-cells calculating unit that calculates the number of cells within the culture container based on the number of cells counted by the number-of-cells counting unit.

公开(公告)号：US10385300B2

公开(公告)日：2019-08-20

申请号：US15653967

申请日：2017-07-19

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu Iga ,  Yohei Tanikawa ,  Shinichi Takimoto ,  Yoshinobu Akahori

IPC: G06K9/00 ,  C12M1/34 ,  G01N21/88 ,  C12M1/36 ,  G01N21/01

Abstract: A cell analysis apparatus including: a cell image acquisition unit that acquires an image of cells in a culture vessel in which cells are cultured; a smoothing processing unit that smooths luminance values in the image acquired by the cell image acquisition unit; a minimum-value detecting unit that detects minimum values of the luminance values smoothed by the smoothing processing unit; a smallest-minimum-value extracting unit that extracts smallest minimum values which are the smallest in regions according to the sizes of the cells, from the minimum values detected by the minimum-value detecting unit; a counting unit that counts the number of smallest minimum values extracted by the smallest-minimum-value extracting unit; and a cell-count calculating unit that calculates the number of cells in the culture vessel on the basis of the number of smallest minimum values counted by the counting unit.

公开(公告)号：US20180092594A1

公开(公告)日：2018-04-05

申请号：US15831675

申请日：2017-12-05

Applicant: OLYMPUS CORPORATION ,  SARATOV State University

Inventor： Yohei Tanikawa ,  Yasunobu Iga ,  Shinichi Takimoto ,  Valery V. Tuchin ,  Elina A. Genina ,  Alexey N. Bashkatov ,  Irina Yu. Yanina ,  Yana V. Tarakanchikova ,  Georgy S. Terentuk ,  Polina A. Timoshina ,  Daria K. Tuchina

IPC: A61B5/00

CPC classification number: A61B5/489 ,  A61B5/0082 ,  A61B5/0086 ,  A61B5/4872 ,  A61B2505/05

Abstract: A fat tissue observation method includes: a process A of applying an optical clearing agent to fat tissue within a body; a process B of heating the fat tissue to reduce a first refractive index of the fat tissue and put the first refractive index close to a second refractive index of the optical clearing agent; and a process C of observing an inside of the fat tissue that the optical clearing agent permeates.