公开(公告)号：US12174105B2

公开(公告)日：2024-12-24

申请号：US18379775

申请日：2023-10-13

Applicant: MIFTEK CORPORATION

Inventor： Masanobu Yamamoto ,  J. Paul Robinson ,  Keegan Hernandez

IPC: G01N15/14 ,  G01N15/1429 ,  G01N15/1434

Abstract: A system is described wherein a plurality of lasers are used to irradiate particles in a flow cytometer's flow stream. In certain embodiments, a light source having a plurality of lasers configured for irradiation of a flow stream are disclosed where discrete intervals of irradiation by one or more discretely activated lasers are triggered by irradiation of one or more particles in the flow stream with one or more continuously on lasers.

公开(公告)号：US12050130B2

公开(公告)日：2024-07-30

申请号：US18542717

申请日：2023-12-17

Applicant: MIFTEK CORPORATION

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson ,  John Jaiber González Murillo

IPC: G01J3/02 ,  G01J3/44

CPC classification number: G01J3/4406 ,  G01J3/0218 ,  G01J3/0227

Abstract: A measurement system is disclosed which includes a vessel configured to suspend molecules of interest therein, optical sources configured to excite the molecules of interest by an excitation light activated and deactivated in a stepwise fashion, during the activation, the sources activated according to a pulse train, the molecules of interest emitting emission light in response to being excited by the excitation light, one or more sensor packages each comprising a plurality of photodetectors configured to receive emission light from the molecules of interest and, in response, provide an output voltage signal and an output current signal corresponding to photoelectron response of an incident photon on the one or more sensor packages, and a detector configured to determine successive single molecular decay of the molecules of interest, generate an emission pulse associated with each incident photon on the one or more sensor packages, and count the number of emission pulses.

公开(公告)号：US20240133740A1

公开(公告)日：2024-04-25

申请号：US18542717

申请日：2023-12-17

Applicant: MIFTEK CORPORATION

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson ,  John Jaiber González Murillo

IPC: G01J3/44 ,  G01J3/02

CPC classification number: G01J3/4406 ,  G01J3/0218 ,  G01J3/0227

Abstract: A measurement system is disclosed which includes a vessel configured to suspend molecules of interest therein, optical sources configured to excite the molecules of interest by an excitation light activated and deactivated in a stepwise fashion, during the activation, the sources activated according to a pulse train, the molecules of interest emitting emission light in response to being excited by the excitation light, one or more sensor packages each comprising a plurality of photodetectors configured to receive emission light from the molecules of interest and, in response, provide an output voltage signal and an output current signal corresponding to photoelectron response of an incident photon on the one or more sensor packages, and a detector configured to determine successive single molecular decay of the molecules of interest, generate an emission pulse associated with each incident photon on the one or more sensor packages, and count the number of emission pulses.

公开(公告)号：US11852537B2

公开(公告)日：2023-12-26

申请号：US18130867

申请日：2023-04-04

Applicant: MIFTEK CORPORATION

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson ,  John Jaiber González Murillo

IPC: G01J3/02 ,  G01J3/44

CPC classification number: G01J3/4406 ,  G01J3/0218 ,  G01J3/0227

Abstract: A flow cytometry measurement system is disclosed which includes a flow chamber configured to flow particles of interest in a flow stream, one or more optical sources configured to excite the particles of interest by an excitation light activated and deactivated according to a pulse train thus causing particles of interest emitting emission light, one or more sensor packages each comprising a plurality of photodetectors configured to receive emission light from the particles of interest and, in response, provide an output voltage signal and an output current signal corresponding to photoelectron response of an incident photon on the one or more sensor packages, and a detector configured to determine successive single molecular decay of the particles of interest, generate an emission pulse associated with each incident photon on the one or more sensor packages, and count the number of emission pulses.