公开(公告)号：US20240002910A1

公开(公告)日：2024-01-04

申请号：US18465623

申请日：2023-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11254968B2

公开(公告)日：2022-02-22

申请号：US15688250

申请日：2017-08-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US10155207B2

公开(公告)日：2018-12-18

申请号：US15171616

申请日：2016-06-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yevgeny Yurkovetsky ,  Darren R. Link ,  Jonathan William Larson

IPC: B01L3/00 ,  B01F13/00 ,  B01F5/04 ,  B01L7/00 ,  B01F3/08 ,  B01F5/00

Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

公开(公告)号：US20200225129A1

公开(公告)日：2020-07-16

申请号：US16733137

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren Roy Link ,  Mark Weary

IPC: G01N1/38 ,  B01L3/02 ,  B01L3/00 ,  B01F15/00 ,  B01F13/00 ,  F17D3/01 ,  G01N27/447 ,  F16K99/00

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US20190134581A1

公开(公告)日：2019-05-09

申请号：US16181256

申请日：2018-11-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yevgeny Yurkovetsky ,  Darren Roy Link ,  Jonathan William Larson

IPC: B01F13/00 ,  B01L7/00 ,  B01L3/00 ,  B01F3/08 ,  B01F5/00 ,  B01F5/04

Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.