公开(公告)号：US11110458B2

公开(公告)日：2021-09-07

申请号：US14171766

申请日：2014-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： George Carman ,  Thomas H. Cauley, III ,  David P. Stumbo

IPC: C12Q1/68 ,  B01L3/00

Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.

公开(公告)号：US10551603B2

公开(公告)日：2020-02-04

申请号：US15656877

申请日：2017-07-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Denis Pristinski ,  David P. Stumbo

IPC: G02B19/00 ,  G01N35/08 ,  G01N15/14 ,  G01N21/64 ,  F21V8/00 ,  G01N15/10 ,  G02B6/26

Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.

公开(公告)号：US10060847B2

公开(公告)日：2018-08-28

申请号：US15394624

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yunfeng Ling ,  David P. Stumbo ,  George Carman ,  Denis Pristinski

IPC: G01N15/14

CPC classification number: G01N15/1429 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1461 ,  G01N2015/1488 ,  G01N2021/4707 ,  G01N2021/4728

Abstract: Systems and methods for detecting and processing signals from particles. In an exemplary method, particles may be passed through a zone of a channel, while the zone is irradiated with light. Interaction of the light with the particles may deflect light and induce photoluminescence. A deflection signal and a photoluminescence signal may be detected from the zone. Particle waveforms may be identified in the deflection signal. At least a subset of the particle waveforms may be double-peak waveforms including a pair of peaks corresponding to a particle entering and exiting the zone. Amplitudes may be obtained from the photoluminescence signal. The amplitudes may correspond to respective particles and their particle waveforms, and at least a subset of the amplitudes may correspond to the double-peak waveforms. Individual particles may be assigned as positive or as negative for an analyte based on the corresponding amplitudes.