公开(公告)号：US11630067B2

公开(公告)日：2023-04-18

申请号：US17124285

申请日：2020-12-16

Applicant: Applied Materials, Inc.

Inventor： Yun-Ching Chang ,  Dan Xie ,  Chloe Kim

IPC: C12Q1/6841 ,  G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/36 ,  G06T5/00 ,  G06T5/20 ,  G06T5/50 ,  G06T7/00 ,  G16B50/40 ,  G06V10/30 ,  G06V10/75 ,  G06V20/69 ,  G01N21/75

Abstract: In a fluorescent in-situ hybridization imaging system performs, as nested loops, the following: (1) a valve sequentially couples a flow cell to a plurality of different reagent sources to expose the sample to a plurality of different reagents, (2) for each reagent of the plurality of different reagents, a motor sequentially positions the fluorescence microscope relative to sample at a plurality of different fields of view, (3) for each field of view of the plurality of different fields of view, a variable frequency excitation light source sequentially emits a plurality of different wavelengths, (4) for each wavelength of the plurality of different wavelengths, an actuator sequentially positions the fluorescence microscope relative to sample at a plurality of different vertical heights, and (5) for each vertical height of the plurality of different vertical heights, an image is obtained.

公开(公告)号：US20220066190A1

公开(公告)日：2022-03-03

申请号：US17461827

申请日：2021-08-30

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: G02B21/34 ,  G01N21/64

Abstract: A calibration slide for calibrating a multiplexed fluorescence in-situ hybridization (FISH) system has a substrate having a surface and a first plurality of beads on the surface. Each bead of the first plurality of beads has at least one binding domain to be bound to a corresponding probe that has a tag and a targeting domain that binds to a binding domain.

公开(公告)号：US20220065787A1

公开(公告)日：2022-03-03

申请号：US17460052

申请日：2021-08-27

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: G01N21/64 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: This disclosure relates to methods for image registration in fluorescence assays for the detection and quantitation of analytes in a sample using functionalized alignment beads as fiducial markers to improve image registration.

公开(公告)号：US20220064718A1

公开(公告)日：2022-03-03

申请号：US17460056

申请日：2021-08-27

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: C12Q1/6837 ,  C12Q1/6818

Abstract: Functionalized alignment beads each have a plurality of binding sites, the plurality binding sites including a first subset and a second subset of binding sites. The first subset of binding sites include a first nucleotide sequence selected to bind to a complementary first nucleotide sequence of a first probe that has a fluorophore and that targets the first nucleotide sequence in a sample or in a first targeting probe that targets the sample. The second subset of the plurality of binding sites include a second nucleotide sequence selected to bind to a complementary second nucleotide sequence of a second probe that has a fluorophore and that targets the second nucleotide sequence in a sample or in a second targeting probe that targets the sample. The fluorophore of the first probe has a same emission wavelength and/or same excitation wavelength as the fluorophore of the second probe.

公开(公告)号：US20210183034A1

公开(公告)日：2021-06-17

申请号：US17124305

申请日：2020-12-16

Applicant: Applied Materials, Inc.

Inventor： Yun-Ching Chang ,  Dan Xie ,  Chloe Kim

IPC: G06T5/50 ,  G06T5/00 ,  G06T7/00 ,  G06T5/20 ,  G02B21/16 ,  G02B21/36 ,  G01N21/64 ,  C12Q1/6841

Abstract: A fluorescent in-situ hybridization imaging and analysis system includes a flow cell to contain a sample to be exposed to fluorescent probes in a reagent, a fluorescence microscope to obtain sequentially collect a plurality of images of the sample at a plurality of different combinations of imaging parameters, and a data processing system. The data processing system includes an online pre-processing system configured to sequentially receive the images from the fluorescence microscope as the images are collected and perform on-the-fly image pre-processing to remove experimental artifacts of the image and to provide RNA image spot sharpening, and an offline processing system configured to, after the plurality of images are collected, perform registration of images having a same field of view and to decode intensity values in the plurality of images to identify expressed genes.