公开(公告)号：US11642608B2

公开(公告)日：2023-05-09

申请号：US17346329

申请日：2021-06-14

Applicant: Ziheng Jin ,  Sujing Gu ,  Yanjun Wen ,  Linzheng Li

Inventor： Ziheng Jin ,  Sujing Gu ,  Yanjun Wen ,  Linzheng Li

IPC: B01D11/02 ,  B01D21/26 ,  B01D37/00 ,  A61K36/48 ,  B01D11/04 ,  A61K36/482 ,  C07D311/28

CPC classification number: B01D11/0288 ,  A61K36/482 ,  B01D11/0292 ,  B01D11/048 ,  B01D21/262 ,  B01D37/00 ,  A61K2236/33 ,  A61K2236/51 ,  A61K2236/53 ,  C07D311/28

Abstract: A method for preparing a Hovenia dulcis Thunb extract rich in dihydromyricetin includes the following steps: (1) crushing Hovenia dulcis Thunb seeds to obtain a Hovenia dulcis Thunb powder; (2) adding a 10-95% ethanol solution in an amount of 3-15 times of an amount of the Hovenia dulcis Thunb powder, stirring and extracting at 20° C.-80° C. twice; (3) filtering to obtain an extract solution; (4) concentrating the extract solution by evaporating ethanol under reduced pressure to obtain a crude extract, the crude extract having a solid content of 10%-40%; (5) placing the crude extract at −20° C. to 8° C. for 0.5 to 12 hours; (6) centrifuging the crude extract to obtain a supernatant; and (7) spray-drying the supernatant to obtain the Hovenia dulcis Thunb extract.

公开(公告)号：US12012627B2

公开(公告)日：2024-06-18

申请号：US18154827

申请日：2023-01-15

Applicant: Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

Inventor： Sanying Wang ,  Linzheng Li ,  Ziheng Jin ,  Yanjun Wen

IPC: C12P19/24 ,  C12N1/20 ,  C12N9/90 ,  C12N15/52 ,  C12N15/75 ,  C12R1/125

CPC classification number: C12P19/24 ,  C12N1/205 ,  C12N9/90 ,  C12N15/52 ,  C12N15/75 ,  C12N2500/02 ,  C12N2500/34 ,  C12N2500/74 ,  C12N2523/00 ,  C12R2001/125 ,  C12Y501/03

Abstract: The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

公开(公告)号：US11654378B2

公开(公告)日：2023-05-23

申请号：US17352295

申请日：2021-06-19

Applicant: Sujing Gu ,  Ziheng Jin ,  Yanjun Wen ,  Linzheng Li

Inventor： Sujing Gu ,  Ziheng Jin ,  Yanjun Wen ,  Linzheng Li

IPC: B01D11/02 ,  C07C50/18 ,  C08B37/08 ,  B01D11/04 ,  B01D61/14 ,  C08B37/00

CPC classification number: B01D11/0288 ,  B01D11/0284 ,  B01D11/0415 ,  B01D11/0492 ,  B01D61/145 ,  C07C50/18 ,  C08B37/0087

Abstract: A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.