Method and kit for calibrating a photoluminescence measurement
    11.
    发明申请
    Method and kit for calibrating a photoluminescence measurement 有权
    用于校准光致发光测量系统的方法和试剂盒

    公开(公告)号:US20060214112A1

    公开(公告)日:2006-09-28

    申请号:US11223202

    申请日:2005-09-09

    IPC分类号: G01J1/58 G01N31/00

    摘要: The invention is directed to a method and a kit for calibrating a photoluminescence measurement system, in particular a fluorescence measurement system. The kit includes a number of fluorescence standards i and their corrected and certified fluorescence spectra Ii(λ), whereby the fluorescence standards i are selected, so that their spectrally corrected fluorescence spectra Ii(λ) cover a broad spectral range with high intensity. The standards are characterized by large half-widths FWHMi of their bands of at least 1400 cm−1. According to the method of the invention, partial correction functions Fi(λ) are generated by forming the quotient of the measured fluorescence spectra Ji(λ) and the corresponding corrected fluorescence spectra Ii(λ), which are then combined to form a total correction function F(λ) for a broad spectral range. The combination factors αi are hereby computed by statistical averaging of consecutive partial correction functions Fi(λ) over only a predefined, limited overlap region λi/i+1±ΔλOL about the mutual crossover wavelength λi/i+1.

    摘要翻译: 本发明涉及用于校准光致发光测量系统,特别是荧光测量系统的方法和试剂盒。 该试剂盒包括许多荧光标准品i及其校准和认证的荧光光谱Iλ(λ),由此选择荧光标准品i,使得它们的光谱校正的荧光光谱I i < / SUB(λ)覆盖了具有高强度的宽光谱范围。 这些标准的特征在于它们的至少1400cm -1的波段的大半宽度FWHMi 。 根据本发明的方法,通过形成所测量的荧光光谱λ(λ)的商和对应的校正函数来产生部分校正函数F(λ) 荧光光谱I(λ),然后将其组合以形成宽光谱范围的总校正函数F(λ)。 因此,通过仅在预定义的有限重叠区域λi / i上的连续部分校正函数F(i)的统计平均来计算组合因子αi i i 关于相互交叉波长λ1/ i + 1的+1 + / / / / -