公开(公告)号：US11861514B2

公开(公告)日：2024-01-02

申请号：US17016244

申请日：2020-09-09

申请人： Luminex Corporation

发明人： Bryan Richard Davidson ,  Vidya Venkatachalam ,  Artiom Zayats ,  Michael C. Riedel

IPC分类号： G06N5/04 ,  G06N20/00

CPC分类号： G06N5/04 ,  G06N20/00

摘要： A computer system is configured to receive a dataset of image-derived features for a plurality of images, reduce the dimensionality of this dataset, identify clusters within the dimensionally-reduced dataset, and generate a visual representation of the datapoint of the dimensionally-reduced dataset as icons grouped by cluster. User input is received to apply user classification labels to the images for inclusion in a training dataset. A user interface is useable to present information to the user and receive information from the user to facilitate the application of user classification labels.

公开(公告)号：US20220364148A1

公开(公告)日：2022-11-17

申请号：US17808180

申请日：2022-06-22

申请人： LUMINEX CORPORATION

发明人： Douglas WHITMAN ,  Jennifer BERNIER ,  William WANG

IPC分类号： C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6823

摘要： Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.

公开(公告)号：US20220282307A1

公开(公告)日：2022-09-08

申请号：US17824215

申请日：2022-05-25

申请人： LUMINEX CORPORATION

发明人： Doug WHITMAN

IPC分类号： C12Q1/6818 ,  C12Q1/6876

摘要： Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.

公开(公告)号：US20220251633A1

公开(公告)日：2022-08-11

申请号：US17709693

申请日：2022-03-31

申请人： LUMINEX CORPORATION

发明人： Douglas F. WHITMAN ,  Hongwei ZHANG

IPC分类号： C12Q1/6818 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6853

摘要： Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

公开(公告)号：US11261481B2

公开(公告)日：2022-03-01

申请号：US16804060

申请日：2020-02-28

申请人： LUMINEX CORPORATION

发明人： Doug Whitman ,  Nicolas Arab ,  Chuck Collins

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/6818

摘要： Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

公开(公告)号：US20210189469A1

公开(公告)日：2021-06-24

申请号：US17196200

申请日：2021-03-09

申请人： LUMINEX CORPORATION

发明人： Scott C. JOHNSON ,  Nicolas ARAB ,  Doug WHITMAN

IPC分类号： C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823

摘要： Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US11001877B2

公开(公告)日：2021-05-11

申请号：US16284043

申请日：2019-02-25

申请人： LUMINEX CORPORATION

发明人： Douglas F. Whitman ,  Charles J. Collins

IPC分类号： C12Q1/68 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q1/6804

摘要： The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process.

公开(公告)号：US20210054444A1

公开(公告)日：2021-02-25

申请号：US16994763

申请日：2020-08-17

申请人： LUMINEX CORPORATION

发明人： Edward A. SEKINGER ,  Megan MARTINEZ ,  Mahima PANCHOLI ,  Kurt HOFFACKER

IPC分类号： C12Q1/6844 ,  C12Q1/6806

摘要： Provided herein are methods for the rapid preparation of amplifiable nucleic acids from biological samples, which can be applied to various applications, such as, for example, point-of-care diagnostics, service laboratory diagnostics, and molecular biology applications. These methods can be performed in 15 minutes or less, and preferably in 5 minutes or less. For most applications, no further purification of nucleic acids is needed.

公开(公告)号：US20200263240A1

公开(公告)日：2020-08-20

申请号：US16869758

申请日：2020-05-08

申请人： LUMINEX CORPORATION

发明人： Martin KOCHANCZYK ,  Doug WHITMAN

IPC分类号： C12Q1/6827

摘要： Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US10733418B2

公开(公告)日：2020-08-04

申请号：US15987359

申请日：2018-05-23

申请人： Luminex Corporation

发明人： Matthew S. Fisher ,  Nicolas Arab

IPC分类号： G06K9/00 ,  G01N15/10 ,  G01N15/14

摘要： An apparatus and method for image normalization using a Gaussian residual of fit selection criteria. The method may include acquiring a two-dimensional image of a plurality of particles, where the plurality of particles comprises a plurality of calibration particles, and identifying a calibration particle by correlating a portion of the image corresponding to the calibration particle to a mathematical model (e.g. Gaussian fit). The measured intensity of the calibration particle may then be used to normalize the intensity of the image.