METHODS AND KITS FOR MULTIPLEX AMPLIFICATION OF SHORT TANDEM REPEAT LOCI
    4.
    发明申请
    METHODS AND KITS FOR MULTIPLEX AMPLIFICATION OF SHORT TANDEM REPEAT LOCI 审中-公开
    用于短暂放样的多重放大的方法和工具

    公开(公告)号:US20150037791A1

    公开(公告)日:2015-02-05

    申请号:US14328074

    申请日:2014-07-10

    Abstract: Compositions, methods and kits are disclosed for use in simultaneously amplifying at least 20 specific STR loci of genomic nucleic acid in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 23 and 24 specific loci in a single multiplex reaction, comprising the 13 CODIS loci, the Amelogenin locus, an InDel and at least six to ten additional STR loci, including methods, kits and materials for the analysis of these loci.

    Abstract translation: 公开了用于在单个多重反应中同时扩增基因组核酸的至少20个特异性STR基因座的组合物,方法和试剂盒,以及用于分析这些反应的产物的方法和材料。 本发明包括用于在单个多重反应中同时扩增23和24个特异性基因座的材料和方法,包括13个CODIS基因座,Amelogenin基因座,InDel和至少6至10个另外的STR基因座,包括方法, 用于分析这些基因座的试剂盒和材料。

    FAST PCR FOR STR GENOTYPING
    5.
    发明申请

    公开(公告)号:US20230099446A1

    公开(公告)日:2023-03-30

    申请号:US17950990

    申请日:2022-09-22

    Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

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