公开(公告)号：US20160264950A1

公开(公告)日：2016-09-15

申请号：US14835609

申请日：2015-08-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Rogers ,  Bianca Lam ,  Joanna Guo ,  Lushen Li

IPC: C12N9/12 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12N9/12 ,  C12N9/99 ,  C12P19/34 ,  C12Y207/07049

Abstract: The present invention provides reversibly inactivated reverse transcriptase enzymes, particularly those having increased thermostability and/or thermoreactivity and compositions, methods and kits that include such enzymes, for the reverse transcription of nucleic acid molecules. Also provided are compositions and methods for the reactivation of reversibly inactivated reverse transcriptases. More particularly, the present invention relates to compositions and methods that can increase the speed of reactivation and stabilize reactivation of chemically modified reverse transcriptase enzymes prior to, or as part of a reverse transcription reaction. As compared to existing compositions and methods, the reversibly inactivated reverse transcriptase enzymes of the present invention provide for a significant reduction in non-specific reverse transcription from template nucleic acid molecules.

Abstract translation: 本发明提供了可逆地灭活的逆转录酶，特别是那些具有增加的热稳定性和/或热反应性的组合物，包含这些酶的组合物，方法和试剂盒，用于逆转录核酸分子。 还提供了用于重新激活可逆失活的逆转录酶的组合物和方法。 更具体地说，本发明涉及可以在逆转录反应之前或作为逆转录反应的一部分期间增加再活化速度并稳定化学修饰的逆转录酶的再活化的组合物和方法。 与现有的组合物和方法相比，本发明的可逆失活的逆转录酶提供了模板核酸分子的非特异性逆转录的显着降低。