公开(公告)号：US20220343467A1

公开(公告)日：2022-10-27

申请号：US17636035

申请日：2020-10-08

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis ALVAREZ ,  Frank HECHT

IPC: G06T5/00 ,  G06T5/50 ,  G06T5/20 ,  G06T5/10 ,  G01N21/64

Abstract: The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.

公开(公告)号：US20230344447A1

公开(公告)日：2023-10-26

申请号：US18026644

申请日：2021-09-20

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank HECHT

IPC: H03M7/30

CPC classification number: H03M7/6011 ,  H03M7/6005 ,  H03M7/3064 ,  H03M7/3077

Abstract: A computer-implemented method for compression of Time Tagged data including Time Tagged data records includes the step of separating the Time Tagged data records into a plurality of groups. The method also includes sorting the Time Tagged data records in at least one of the groups by a photon arrival time. The method also includes subtracting a content of a record by a content of an adjacent record resulting in modified records. The method also includes compressing the modified records with a compression method.

公开(公告)号：US20190339201A1

公开(公告)日：2019-11-07

申请号：US16304168

申请日：2017-05-24

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Bernd WIDZGOWSKI ,  Frank HECHT

IPC: G01N21/64

Abstract: A fluorescence lifetime imaging microscopy method with time-correlated single photon counting includes periodically exciting a sample to emit fluorescence photons, with a measurement interval being defined between each two successive excitation light pulses. A value characterizing fluorescence decay behavior is determined based on detection times of detected fluorescence photons, and imaging is performed based one the value. An analog detector signal is sampled within a plurality of sampling intervals within a respective one of the measurement intervals and is converted into a sequence of discrete signal values associated with the sampling intervals. It is determined based thereon whether more than a predefined number of fluorescence photons has been detected within the respective measurement interval. If more than the predefined number of fluorescence photons has been detected, the respective measurement interval is discarded in the step of determining the value characterizing the fluorescence decay behavior.

公开(公告)号：US20240411123A1

公开(公告)日：2024-12-12

申请号：US18705602

申请日：2021-11-02

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank SIECKMANN ,  Frank HECHT

IPC: G02B21/36

Abstract: A method for providing position information for retrieving a target position in a microscopic sample includes providing a first representation of the sample at a first resolution including the target position; specifying a first target position identifier indicating the target position at the first resolution; acquiring an image stack comprising the target position indicated by the first target position identifier; providing a second representation at a second resolution higher than the first resolution based on the image stack; specifying a second target position identifier indicating the target position at the second resolution; specifying a plurality of reference position identifiers in the second representation indicating positions of optically detectable reference markers at the second resolution; and determining a set of geometric descriptors describing spatial relations between the second target position identifier and the plurality of reference position identifiers to provide the position information.

公开(公告)号：US20240290117A1

公开(公告)日：2024-08-29

申请号：US18586622

申请日：2024-02-26

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis ALVAREZ ,  Frank HECHT ,  Julia ROBERTI ,  Giulia OSSATO

IPC: G06V20/69 ,  G01N21/64 ,  G06V10/25

CPC classification number: G06V20/698 ,  G01N21/6408 ,  G01N21/6458 ,  G06V10/25 ,  G06V20/693 ,  G06V20/695

Abstract: A processor for lifetime-based unmixing in fluorescence microscopy is configured to acquire an image having a plurality of pixels, each pixel providing information on photon count and photon arrival times, generate a phasor plot that is a vector space representation of the image, partition the image into image segments, evaluate the image segments according to total photon counts of the corresponding subsets of pixels, and execute a lifetime classification by selecting an image segment having a largest total photon count, determining a region of interest in the image encompassing the image segment, determining a phasor subset in the phasor plot corresponding to the region of interest, and generating a lifetime class including a set of image segments corresponding to the phasor subset. A plurality of lifetime classes is generated by iteratively executing the lifetime classification. The processor is configured to perform lifetime-based unmixing using the life-time classes.

公开(公告)号：US20240210283A1

公开(公告)日：2024-06-27

申请号：US18390004

申请日：2023-12-20

Applicant: Leica Mikrosysteme GmbH ,  Leica Microsystems CMS GmbH

Inventor： Frank HECHT ,  Paul WURZINGER ,  Matthias KATZENGRUBER

IPC: G01N1/06

CPC classification number: G01N1/06 ,  G01N2001/065

Abstract: A controller for a microtome configured to cut slices from a sample block is configured to control a display to visualize a virtual representation of the sample block, receive first user input data indicative of a front surface of the sample block, determine a virtual front surface of the sample block based on the first user input data, receive second user input data indicative of an edge of the front surface of the sample block, determine a virtual edge of the front surface of the sample block based on the second user input data, receive third user input data indicative of a cutting plane intersecting the sample block, determine a virtual cutting plane intersecting the sample block based on the third user input, and determine alignment parameters based on the virtual front surface and the virtual edge of the front surface of the sample block and the virtual cutting plane.