公开(公告)号：US20230272319A1

公开(公告)日：2023-08-31

申请号：US18015791

申请日：2021-05-28

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA

IPC: C12M1/00

CPC classification number: C12M23/02

Abstract: An exposure unit is used in order to expose a gas-phase substance to a cell in a buffer solution. The exposure unit includes a base body. The base body has a groove formed in the base body so as to be exposed to an outside and retaining the cell together with the buffer solution, and a first storage portion connected to one end of the groove and storing the buffer solution to be supplied to the groove.

公开(公告)号：US20220155297A1

公开(公告)日：2022-05-19

申请号：US17423133

申请日：2019-11-06

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Sayaka KAZAMI ,  Yu HASHIMOTO ,  Hiroyasu ITOH

IPC: G01N33/566 ,  G01N33/533 ,  G01N21/78 ,  C12M1/40

Abstract: A method for selecting cells highly responsive to a target substance according to the present invention comprises step of: (1a) bringing a sample containing a target substance into contact with a plurality of cells, wherein the cells are cells having a receptor for the target substance and a fluorescent indicator, and the fluorescent indicator emits fluorescence as a result of binding between the target substance and the receptor; (1b) calculating a fluorescence intensity increase rate for each of the cells; and (1c) selecting arbitrary cells exhibiting a fluorescence intensity increase rate within the top 50% among the plurality of cells. According to the present invention, it is possible to detect the target substance in the sample with high sensitivity.

公开(公告)号：US20220187240A1

公开(公告)日：2022-06-16

申请号：US17547377

申请日：2021-12-10

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Kazuya SUZUKI ,  Ryo MAKINO ,  Hiroyasu ITOH

IPC: G01N27/447 ,  G01N21/64 ,  B03C5/00 ,  B03C5/02

Abstract: A microparticle trapping device includes: a fluid channel configured to be injected with a fluid including a microparticle; first and second electrodes configured to generate an electric field in the fluid channel; and an electrical insulator formed with at least one opening between the first and second electrodes in the fluid channel. The electrical insulator is disposed between the first and second electrodes so that an inhomogeneous electric field is made through the at least one opening between the first and second electrodes in the fluid channel, and the still other aspect is configured to trap the microparticle through dielectrophoresis.

公开(公告)号：US20220120271A1

公开(公告)日：2022-04-21

申请号：US17310814

申请日：2020-02-19

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Hiroyasu ITOH

IPC: F04B53/16 ,  F04B53/10 ,  F04B43/04 ,  C12M1/00

Abstract: In a liquid supply device, the pump unit has a discharge port for discharging a fluid. The connecting portion is connected to the discharge port. The first and second pipe portions and branch off from the connecting portion. The first introduction unit is connected to the first pipe portion and introduces a first liquid into the first flow channel at a flow rate corresponding to a flow rate of the fluid flowing through the first pipe portion. The second introduction unit is connected to the second pipe portion and introduces a second liquid into the first flow channel at a flow rate corresponding to a flow rate of the fluid flowing through the second pipe portion. The stopping portion stops the flow of the fluid in the second pipe portion.