公开(公告)号：US12291712B2

公开(公告)日：2025-05-06

申请号：US17307877

申请日：2021-05-04

Applicant: Genentech, Inc.

Inventor： Karthik Veeravalli ,  Rebekah McKenna

IPC: C12N15/70 ,  C07K16/00 ,  C07K16/22 ,  C07K16/24 ,  C12N9/02 ,  C12N9/90

Abstract: Provided herein are methods and host cells for producing a polypeptide containing two chains, such as an antibody, half-antibody, antibody fragment, or one-armed antibody. The methods and host cells allow for two-chain polypeptide production using expression of polynucleotides encoding the polypeptide chains from extra-chromosomal polynucleotide(s), and expression of one or more chaperone protein(s) (e.g., peptidyl-prolyl isomerases and/or protein disulfide oxidoreductases) from the host cell chromosome using non-native combination(s) of promoters and translational units encoding a chaperone protein.

公开(公告)号：US09850514B2

公开(公告)日：2017-12-26

申请号：US15067646

申请日：2016-03-11

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12N9/10 ,  C07K16/32 ,  C07K16/22 ,  C12P21/02 ,  C07K16/18 ,  C07K16/40 ,  C12N15/70 ,  C07K16/28

CPC classification number: C12P21/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/14 ,  C12N9/1029 ,  C12N9/1085 ,  C12N15/70 ,  C12P21/02 ,  C12Y203/01046 ,  C12Y205/01006

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US10179925B2

公开(公告)日：2019-01-15

申请号：US15816305

申请日：2017-11-17

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/22 ,  C07K16/40 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US11434482B2

公开(公告)日：2022-09-06

申请号：US15782616

申请日：2017-10-12

Applicant: Genentech, Inc.

Inventor： Twyla Noelle Lombana ,  Rebekah McKenna ,  Christoph Spiess ,  Karthik Veeravalli ,  Michael Dillon

IPC: G01N33/53 ,  C12N15/10 ,  C07K16/22 ,  C07K16/24 ,  C07K14/195 ,  C07K16/12 ,  C12Q1/689 ,  G01N33/569 ,  G01N33/68

Abstract: The invention provides methods of identifying bacteria comprising binding polypeptides. The invention also provides methods of identifying bacteria with improved expression of binding polypeptides. The invention also provides methods of identifying binding polypeptides with improved expression. The invention also provides engineered bacteria suitable for use in the methods of the invention. The invention also provides compositions that can be obtained using the methods, for example, anti-interleukin-13 (IL-13) antibodies with improved expression and/or stability. The invention also provides libraries comprising binding polypeptide (e.g., antibody) variants.

公开(公告)号：US11015214B2

公开(公告)日：2021-05-25

申请号：US16536149

申请日：2019-08-08

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C07K16/22 ,  C07K16/18 ,  C07K16/40 ,  C12N9/10 ,  C12N15/70 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US10421984B2

公开(公告)日：2019-09-24

申请号：US15951895

申请日：2018-04-12

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/22 ,  C07K16/40 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US20180080056A1

公开(公告)日：2018-03-22

申请号：US15816305

申请日：2017-11-17

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/40 ,  C07K16/32 ,  C07K16/28 ,  C07K16/22

CPC classification number: C12P21/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/14 ,  C12N9/1029 ,  C12N9/1085 ,  C12N15/70 ,  C12P21/02 ,  C12Y203/01046 ,  C12Y205/01006

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US20160186227A1

公开(公告)日：2016-06-30

申请号：US15067646

申请日：2016-03-11

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12N9/10 ,  C07K16/32 ,  C07K16/22 ,  C07K16/40

CPC classification number: C12P21/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/14 ,  C12N9/1029 ,  C12N9/1085 ,  C12N15/70 ,  C12P21/02 ,  C12Y203/01046 ,  C12Y205/01006

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Abstract translation: 本发明涉及用于在细菌中重组蛋白质生产期间防止将正亮氨酸掺入蛋白质的方法和组合物。 本发明还提供了与本文提供的方法和组合物一起使用的微生物宿主细胞和核酸分子。

公开(公告)号：US20140081003A1

公开(公告)日：2014-03-20

申请号：US14031463

申请日：2013-09-19

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/02 ,  C07K16/18 ,  C07K16/40 ,  C07K16/22

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Abstract translation: 本发明涉及用于在细菌中重组蛋白质生产期间防止将正亮氨酸掺入蛋白质的方法和组合物。 本发明还提供了与本文提供的方法和组合物一起使用的微生物宿主细胞和核酸分子。