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    VARIANT CLASSIFICATION THROUGH HIGH-CONFIDENCE MUTATION DETECTION FROM FLUORESCENCE SIGNALS MEASURED WITH A MULTIPLE MUTATION ASSAY
    1.
    发明公开
    VARIANT CLASSIFICATION THROUGH HIGH-CONFIDENCE MUTATION DETECTION FROM FLUORESCENCE SIGNALS MEASURED WITH A MULTIPLE MUTATION ASSAY 审中-公开

    公开(公告)号：US20240076751A1

    公开(公告)日：2024-03-07

    申请号：US18234573

    申请日：2023-08-16

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Steven Okino

    IPC: C12Q1/70 ,  G01N33/58

    CPC classification number: C12Q1/70 ,  G01N33/582 ,  G01N2800/26

    Abstract: A system and method for SARS-CoV-2 variant classification through mutation detection from qPCR fluorescence signals. The system receives fluorescence signals, wherein a first fluorescence signal indicates quantitative presence of a first genomic region as a control for SARS-CoV-2, and a second fluorescence signal indicates quantitative presence of a second genomic region of a first mutation present in a subset of variants of SARS-CoV-2. The system measures a first Cq for the first fluorescence signal and a second Cq for the second fluorescence signal as the signals cross a threshold RFU. The system calculates a delta Cq as a difference between the two. Further, the system identifies a first peak RFU for the first fluorescence signal and a second peak RFU for the second fluorescence signal, and calculates a RFU ratio of the two. The system detects presence of the first mutation based on the delta Cq and/or the RFU ratio.

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