公开(公告)号：US20220260584A1

公开(公告)日：2022-08-18

申请号：US17616366

申请日：2020-06-05

Applicant: AMGEN INC.

Inventor： Pavel BONDARENKO ,  Scott Thomas KUHNS ,  Andrew NICHOLS ,  Gang XIAO ,  Liuqing SHI ,  Jill A. CROUSE-ZEINEDDINI

IPC: G01N33/68 ,  G01N33/543 ,  G01N33/547

Abstract: Provided herein are methods of identifying structures, e.g., attributes, of a therapeutic protein or a target that affect an interaction between the therapeutic protein and the target. In exemplary embodiments, the method comprises: (a) applying a stress to a first sample comprising therapeutic proteins or targets; (b) contacting the first sample with a second sample comprising targets or therapeutic proteins to form a mixture comprising (i) therapeutic protein-target complexes, (ii) unbound therapeutic proteins, and (iii) unbound targets; and (c) separating the mixture into at least two fractions, wherein an unbound fraction comprises unbound therapeutic proteins or unbound targets and a bound fraction comprises therapeutic protein-target complexes; and (d) for each of the unbound fraction and bound fraction, identifying and quantifying the abundance of the structures, e.g., attribute, present on a species of the therapeutic protein or target.

公开(公告)号：US20190233543A1

公开(公告)日：2019-08-01

申请号：US16338292

申请日：2017-09-28

Applicant: AMGEN INC.

Inventor： Neeraj Jagdish AGRAWAL ,  Pavel BONDARENKO ,  Joon Hoi HUH ,  Andrew NICHOLS ,  Da REN ,  Richard SMITH ,  Riki STEVENSON

IPC: C07K16/40 ,  C07K16/28

CPC classification number: C07K16/40 ,  C07K16/2869 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.