公开(公告)号：US08969405B2

公开(公告)日：2015-03-03

申请号：US12999218

申请日：2009-06-22

Applicant: Thomas R. Webb ,  Chandraiah Lagisetti

Inventor： Thomas R. Webb ,  Chandraiah Lagisetti

IPC: C07D493/10 ,  C07D319/06 ,  A61K31/351 ,  A61K31/357

CPC classification number: C07D493/10 ,  A61K31/351 ,  A61K31/357 ,  C07D319/06

Abstract: In one aspect, the invention relates to compounds having anticancer activity; synthetic methods for making the compounds; pharmaceutical compositions comprising the compounds; and methods of treating disorders associated with uncontrolled cellular proliferation using the compounds and compositions. This abstract is intended to be used as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract translation: 一方面，本发明涉及具有抗癌活性的化合物; 制备化合物的合成方法; 包含该化合物的药物组合物; 以及使用所述化合物和组合物治疗与不受控制的细胞增殖相关的疾病的方法。 该摘要旨在用作扫描工具，用于在特定技术中进行搜索，而不意在限制本发明。

公开(公告)号：US08653327B2

公开(公告)日：2014-02-18

申请号：US13178199

申请日：2011-07-07

Applicant: Narasimha C. Samboju ,  Kerrm Y. Yau ,  Frank Burroughs ,  Jayakumar P. Samuel ,  Steven R. Webb

Inventor： Narasimha C. Samboju ,  Kerrm Y. Yau ,  Frank Burroughs ,  Jayakumar P. Samuel ,  Steven R. Webb

IPC: C07K14/415 ,  A01H5/10 ,  A01H1/04 ,  A01H1/02 ,  B82Y30/00 ,  B82Y10/00 ,  B82Y5/00 ,  C12N15/87

CPC classification number: C12N15/8206 ,  B82Y5/00 ,  C12N15/8207 ,  C12N15/8221

Abstract: Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.

Abstract translation: 将目的线性核酸分子引入到包含细胞壁的细胞中的方法包括使用涂覆有聚乙二醇的纳米颗粒。 在一些实施方案中，包含细胞壁的细胞是植物细胞。 方法包括遗传或其他修饰植物和用于治疗或预防包含细胞壁的植物细胞中的疾病。 转基因植物包括由用线性核酸分子转化的植物细胞再生整株植物产生的目的核酸分子。

公开(公告)号：US08609420B2

公开(公告)日：2013-12-17

申请号：US13427750

申请日：2012-03-22

Applicant: Jayakumar P. Samuel ,  Narasimha C. Samboju ,  Kerrm Y. Yau ,  Gaofeng Lin ,  Steven R. Webb ,  Frank Burroughs

Inventor： Jayakumar P. Samuel ,  Narasimha C. Samboju ,  Kerrm Y. Yau ,  Gaofeng Lin ,  Steven R. Webb ,  Frank Burroughs

IPC: C07K14/415 ,  C12N15/87 ,  C12N15/74 ,  A01H5/10 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y5/00

CPC classification number: C12N15/8206 ,  B82Y5/00 ,  C12N15/8207 ,  C12N15/8221

Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Abstract translation: 提供通过使用具有一个或多个细胞穿透肽（CPP）的具有量子点（QD）的QD-肽缀合物将感兴趣的分子引入具有细胞壁的植物细胞的方法。 提供用于遗传或其他修饰植物以及用于治疗或预防包含细胞壁的植物细胞中的疾病的方法。

公开(公告)号：US20130123175A1

公开(公告)日：2013-05-16

申请号：US13535187

申请日：2012-06-27

Applicant: G. Craig Hill ,  Stephen B. Kahl ,  Robert R. Webb ,  Constance A. McKee

Inventor： G. Craig Hill ,  Stephen B. Kahl ,  Robert R. Webb ,  Constance A. McKee

IPC: C07K14/48

CPC classification number: C07K14/48 ,  A61K47/6425 ,  A61K47/6807

Abstract: The invention features compounds of the general formula: B-L-M where B is a binding agent capable of selectively binding to a nerve cell surface receptor and mediating absorption of the compound by the nerve cell; M is a moiety which performs a useful non-cytotoxic function when absorbed by a nerve cell, and can be a therapeutic moiety or an imaging moiety; and L is a linker coupling B to M. The invention also features methods of use of the compounds in, for example, treating conditions such as viral infections and pain, as well as in labeling nerve cells.

Abstract translation: 本发明具有以下通式的化合物：B-L-M其中B是能够选择性结合神经细胞表面受体并介导神经细胞吸收化合物的结合剂; M是当被神经细胞吸收时可以执行有用的非细胞毒性功能的部分，并且可以是治疗部分或成像部分; 并且L是将B连接到M的接头。本发明还涉及在例如治疗病症如病毒感染和疼痛以及标记神经细胞中的化合物的使用方法。

公开(公告)号：US20130042426A1

公开(公告)日：2013-02-21

申请号：US13211364

申请日：2011-08-17

Applicant: SCOTT R. WEBB ,  NICHOLAS W. PINTO, IV ,  PAUL W. ALEXANDER ,  XIUJIE GAO ,  WENDELL G. SUMMERVILLE ,  SCOTT P. CHARNESKY ,  THOMAS W. COX ,  KHRISTOPHER S. LEE

Inventor： SCOTT R. WEBB ,  NICHOLAS W. PINTO, IV ,  PAUL W. ALEXANDER ,  XIUJIE GAO ,  WENDELL G. SUMMERVILLE ,  SCOTT P. CHARNESKY ,  THOMAS W. COX ,  KHRISTOPHER S. LEE

IPC: B60S1/08

CPC classification number: B60S1/0455

Abstract: A vehicle wiper assembly includes a wiper blade configured to wipe a surface, an armature, and an actuator. The armature has a first end spaced from a second end, and is coupled with the wiper blade at the first end, and coupled to a pivot mechanism at the second end. The pivot mechanism is configured to allow the wiper blade to articulate about the second end in a direction substantially away from the surface and between a wiping position and a parked position. The actuator is provided in mechanical communication with the armature and is configured to receive an electrical actuation signal to transition the wiper blade between a wiping position and the parked position, where the wiper blade is in contact with the surface while in the wiping position, and is separated from the surface while in the parked position.

Abstract translation: 车辆刮水器组件包括构造成擦拭表面，电枢和致动器的擦拭器刮片。 衔铁具有与第二端间隔开的第一端，并且在第一端处与雨刮器连接，并且在第二端处联接到枢转机构。 枢转机构构造成允许刮水片在基本上远离表面的方向和擦拭位置与停放位置之间围绕第二端铰接。 致动器设置成与电枢机械连通，并且被配置为接收电致动信号，以在擦拭位置和停放位置之间转移刮水片，其中刮水片在擦拭位置时与表面接触，以及 在停放位置时与表面分离。

公开(公告)号：US08138155B2

公开(公告)日：2012-03-20

申请号：US12749339

申请日：2010-03-29

Applicant: Robert R. Webb ,  Constance A. McKee

Inventor： Robert R. Webb ,  Constance A. McKee

IPC: A61K38/18 ,  A61K47/28 ,  A61K47/42 ,  C07K14/48

CPC classification number: A61K47/6807 ,  A61K47/6425 ,  Y10T436/21

Abstract: A compound for delivering a non-cytotoxic therapeutic moiety into nerve cells, the compound having the general formula: B-L-TM where: B is a binding agent capable of selectively binding to a nerve cell surface receptor and mediating absorption of the compound by the nerve cell; TM is a therapeutic moiety which has a non-cytotoxic therapeutic effect when absorbed by a nerve cell; and L is a linker coupling B to TM.

Abstract translation: 用于将非细胞毒性治疗部分递送到神经细胞中的化合物，具有以下通式的化合物：BL-TM，其中：B是能够选择性结合神经细胞表面受体并通过神经介导化合物的吸收的结合剂 细胞; TM是当被神经细胞吸收时具有非细胞毒性治疗作用的治疗部分; L是连接B到TM的接头。

公开(公告)号：US20110318348A1

公开(公告)日：2011-12-29

申请号：US13124062

申请日：2009-10-13

Applicant: Jianbing Zhang ,  Matthew J. Henry ,  Wangxue Chen ,  Steven R. Webb

Inventor： Jianbing Zhang ,  Matthew J. Henry ,  Wangxue Chen ,  Steven R. Webb

IPC: A61K39/395 ,  A61P31/00 ,  A61P37/08 ,  A61P37/04

CPC classification number: A61K39/395 ,  A61K2039/541 ,  A61K2039/6037 ,  A61K2039/6056 ,  A61K2039/645 ,  C07K16/18 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/92

Abstract: The subject invention provides, for example, a novel approach to specifically induce intranasal and/or oral mucosal as well as humoral antibody response by administrating a mucosal delivery pentabody complex (MDPC). The MDPC is a complex formed by mixing a target antigen and a mucosal delivery pentabody (MDP) that has a strong affinity to the target antigen. The MDP is a fusion protein of a single domain antibody (sdAb; which binds to the target antigen specifically) to a pentamerization domain (which can include the B-subunit of an AB5 toxin family, including the B subunit of cholera toxin (CT) or heat-labile toxin (LT)). The pentamerization domain can self-assemble into a pentamer, through which a pentameric single domain antibody, or a pentabody, is formed.

Abstract translation: 本发明提供了例如通过施用粘膜递送五抗体复合物（MDPC）来特异性诱导鼻内和/或口腔粘膜以及体液抗体应答的新方法。 MDPC是通过混合目标抗原和对靶抗原具有强亲和力的粘膜递送五抗体（MDP）形成的复合物。 MDP是单结构域抗体（sdAb，其特异性结合靶抗原）与五聚体结构域（其可以包括AB5毒素家族的B亚基，包括霍乱毒素（CT）的B亚基）的融合蛋白， 或热不稳定毒素（LT））。 五聚体结构域可以自组装成五聚体，五聚体单结构域抗体或五肽形成。

公开(公告)号：US20110247100A1

公开(公告)日：2011-10-06

申请号：US13042565

申请日：2011-03-08

Applicant: Narasimha C. Samboju ,  Jayakumar P. Samuel ,  Gaofeng Lin ,  Steven R. Webb ,  Frank Burroughs

Inventor： Narasimha C. Samboju ,  Jayakumar P. Samuel ,  Gaofeng Lin ,  Steven R. Webb ,  Frank Burroughs

IPC: A01H5/00 ,  C12N5/04 ,  C12N5/10 ,  C12N9/00

CPC classification number: C12N15/8206

Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.

Abstract translation: 将感兴趣的分子引入具有细胞壁的植物细胞中的方法包括将γ-玉米醇溶蛋白肽与感兴趣的分子相互作用以形成γ-玉米醇溶蛋白连接的结构。 然后将γ-玉米醇溶蛋白连接的结构与具有细胞壁的植物细胞接触，并允许将γ-玉米醇溶蛋白连接的结构摄取到植物细胞中。 或者，通过将γ-玉米醇溶蛋白肽与感兴趣的基因相互作用以形成γ-玉米醇溶蛋白连接的基因结构，可以在具有完整细胞壁的植物细胞中表达感兴趣的基因，从而允许γ-玉米醇溶蛋白连接的基因结构 进入植物细胞，并在植物细胞及其后代中表达感兴趣的基因。

公开(公告)号：US07982006B2

公开(公告)日：2011-07-19

申请号：US12821815

申请日：2010-06-23

Applicant: William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

Inventor： William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

IPC: C07K1/00

CPC classification number: A61K39/08 ,  A61K2039/552 ,  A61K2039/55577 ,  C07K14/33

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract translation: 本发明部分涉及针对坏死性肠炎（NE）的家禽疫苗的开发。 该疫苗使用作为突变的全长无毒产气荚膜梭菌（Cp）α-毒素蛋白（Mcpa）的保护性抗原。 例如，通过减少NE攻击路径中的病变严重程度来证明该疫苗的效用。 本文还公开了用于大量生产该疫苗的新方法。 一个例举的方法涉及在细菌表达系统中产生NE疫苗（突变的α毒素），优选利用荧光假单胞菌系统，用于商业用于控制家禽业中的NE。 本发明的疫苗可以以几种不同的方式优选施用于鸡。 还公开了来自Cp的鸡分离株的新型VI型α毒素。

公开(公告)号：US20090286732A1

公开(公告)日：2009-11-19

申请号：US12323421

申请日：2008-11-25

Applicant: Gordon Craig Hill ,  Stephen B. Kahl ,  Robert R. Webb ,  Constance A. McKee

Inventor： Gordon Craig Hill ,  Stephen B. Kahl ,  Robert R. Webb ,  Constance A. McKee

IPC: A61K38/18 ,  C07K14/475 ,  A61P25/00

CPC classification number: C07K14/48 ,  A61K47/6425 ,  A61K47/6807

Abstract: The invention features compounds of the general formula: B-L-M where B is a binding agent capable of selectively binding to a nerve cell surface receptor and mediating absorption of the compound by the nerve cell; M is a moiety which performs a useful non-cytotoxic function when absorbed by a nerve cell, and can be a therapeutic moiety or an imaging moiety; and L is a linker coupling B to M. The invention also features methods of use of the compounds in, for example, treating conditions such as viral infections and pain, as well as in labeling nerve cells.

Abstract translation: 本发明具有以下通式的化合物：B-L-M其中B是能够选择性结合神经细胞表面受体并介导神经细胞吸收化合物的结合剂; M是当被神经细胞吸收时可以执行有用的非细胞毒性功能的部分，并且可以是治疗部分或成像部分; 并且L是将B连接到M的接头。本发明还涉及在例如治疗病症如病毒感染和疼痛以及标记神经细胞中的化合物的使用方法。