An image analysis method includes acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. The method further includes setting analysis areas to the acquired fluorescent images, calculating a classification value of the analysis areas, classifying the images into one or more groups on the basis of the classification value, calculating an average image of the analysis area every group, subtracting the average image from each image of the analysis area every group to calculate a new image of the analysis area, and calculating a correlation value on the basis of the new images every group.