In a method of manufacturing an offset printing substrate and a method of manufacturing a display substrate, the method includes forming a first coating layer on a base substrate on which is formed a first concave pattern having a first width. An intermediate substrate is also formed upon the first coating layer of the base substrate, the intermediate substrate having a pattern corresponding to the first concave pattern. An offset printing substrate is also formed upon the pattern of the intermediate substrate, the offset printing substrate having a second concave pattern is formed to correspond to the pattern of the intermediate substrate, and the second concave pattern has a second width smaller than the first width.