A method of manufacturing a non-volatile memory device, wherein the method includes: alternately stacking interlayer sacrificial layers and interlayer insulating layers on a substrate; forming a plurality of first openings that pass through the interlayer sacrificial layers and the interlayer insulating layers to expose a first portion of the substrate; forming a semiconductor region on a side wall and a lower surface of each of the first openings; forming an embedded insulating layer in each of the first openings; forming a first conductive layer on the embedded insulating layer inside each of the first openings; forming a second opening exposing a second portion of the substrate and forming an impurity region on the second portion; forming a metal layer to cover the first conductive layer and the impurity region; and forming the metal layer into a metal silicide layer.