发明授权
US08497067B2 Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
有权
从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途
- 专利标题： Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
- 专利标题（中）： 从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途
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申请号： US12087951申请日： 2007-02-26
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公开(公告)号： US08497067B2公开(公告)日： 2013-07-30
- 发明人: Olivier Loudig
- 申请人： Olivier Loudig
- 申请人地址： US NY Bronx
- 专利权人： Albert Einstein College of Medicine of Yeshiva University
- 当前专利权人： Albert Einstein College of Medicine of Yeshiva University
- 当前专利权人地址： US NY Bronx
- 代理机构： Amster, Rothstein & Ebenstein LLP
- 国际申请： PCT/US2007/004892 WO 20070226
- 国际公布： WO2007/103018 WO 20070913
- 主分类号： C12P19/34
- IPC分类号： C12P19/34
摘要：
This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.
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