发明授权
- 专利标题: Method for amplification of nucleotide sequence
- 专利标题(中): 核苷酸序列扩增方法
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申请号: US12375205申请日: 2007-06-20
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公开(公告)号: US07977055B2公开(公告)日: 2011-07-12
- 发明人: Katsunori Nakashima , Isao Ohiso
- 申请人: Katsunori Nakashima , Isao Ohiso
- 申请人地址: JP Hiroshima-shi
- 专利权人: Nishikawa Rubber Co., Ltd.
- 当前专利权人: Nishikawa Rubber Co., Ltd.
- 当前专利权人地址: JP Hiroshima-shi
- 代理机构: Sughrue Mion, PLLC
- 优先权: JP2006-203414 20060726; JP2007-136392 20070523
- 国际申请: PCT/JP2007/062416 WO 20070620
- 国际公布: WO2008/013010 WO 20080131
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C07H21/04
摘要:
To provide a method for selectively amplifying a target nucleic acid and a method for detecting a nucleic acid by the method, which are useful as a method for synthesizing the nucleic acid. A method for amplifying a nucleic acid sequence (EVA (Endonuclease V-dependent Amplification) method) which selectively amplifies a target nucleic acid in a sample, by the use of an oligonucleotide primer containing a base which can be recognized by an endonuclease V, the endonuclease V and a DNA polymerase having a strand displacement activity, and a method for detecting a nucleic acid by the method.
公开/授权文献
- US20100055742A1 METHOD FOR AMPLIFICATION OF NUCLEOTIDE SEQUENCE 公开/授权日:2010-03-04
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