发明授权
- 专利标题: Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group
- 专利标题(中): 使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
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申请号: US11317949申请日: 2005-12-23
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公开(公告)号: US07402390B2公开(公告)日: 2008-07-22
- 发明人: Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
- 申请人: Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
- 申请人地址: KR
- 专利权人: Samsung Electronics Co., Ltd.
- 当前专利权人: Samsung Electronics Co., Ltd.
- 当前专利权人地址: KR
- 代理机构: Cantor Colburn LLP
- 优先权: KR10-2004-0111165 20041223
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N33/53 ; C07H21/00
摘要:
A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
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