发明授权
- 专利标题: Assay for motile facultative anaerobic pathogens
- 专利标题(中): 测定动物厌食病
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申请号: US594647申请日: 1990-10-09
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公开(公告)号: US5187070A公开(公告)日: 1993-02-16
- 发明人: Daniel Y. C. Fung , Linda Yu
- 申请人: Daniel Y. C. Fung , Linda Yu
- 申请人地址: KS Manhattan
- 专利权人: Kansas State University Research Foundation
- 当前专利权人: Kansas State University Research Foundation
- 当前专利权人地址: KS Manhattan
- 主分类号: C12Q1/04
- IPC分类号: C12Q1/04 ; G01N33/04 ; G01N33/12
摘要:
An improved assay for the determination for motile facultative anaerobic pathogens is described which involves the use of a growth-enhancing substance such as oxyrase enzyme in order to increase the growth rate of a target pathogen in a growth medium, to thereby shorten the time required to complete the assay. The assay of the invention is particularly suited for the determination of L. monocytogenes in food products such as meat and dairy goods, and provides and effective screening assay which can be completed in about 24-36 hours. Preferably, a product sample is first incubated in Fraser Broth containing oxyrase enzyme; if a darkened color results, a small portion of the liquid is then placed within a U-shaped culture tube provided with modified Oxford agar; the motility characteristics of the target pathogen are observed in the U-tube, which is indicative of the presence of L. monocytogenes.
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