A microfluidic chip for circulating tumor cell separation, comprising a first shell layer, a second shell layer, and a filter membrane between the first shell layer and the second shell layer. A first channel is formed between the filter membrane and the first shell layer; a second channel is formed between the filter membrane and the second shell layer; the first shell layer is provided with m input interfaces and n output interfaces, wherein m is greater than or equal to 1 and n is greater than or equal to 1; the second shell layer is provided with x input interfaces and y output interfaces, wherein x is greater than or equal to 1 and y is greater than or equal to 1. The chip is used for circulating tumor cell separation to achieve high flux, high efficiency, and a simple method, and facilitate promotion.