Disclosed is an integrated urine anaerobic collection and culture apparatus, comprising: a catheter, at a front end of which a circular arc-shaped guide part and a first through hole are arranged, where a piston part is arranged inside the front end of the catheter; a first branch tube and a second branch tube, which are connected with the catheter, the first branch tube is connected with a first negative pressure vessel, and the first branch tube and the second branch tube are respectively provided with a first valve and a second valve; an aseptic centrifuge tube, which includes a tube body and a first cover body, where the first cover body is provided with a first check valve and a second check valve; a second negative pressure vessel. The present disclosure can realize the whole anaerobic operation of urine collection and culture in the bladder.