The present application provides a display substrate having a plurality of subpixel areas. The display substrate includes a base substrate; a first electrode layer on the base substrate and including a plurality of first electrodes respectively in the plurality of subpixel areas; an auxiliary electrode layer; and an insulating layer between the first electrode layer and the auxiliary electrode layer. The first electrode layer and the auxiliary electrode layer are spaced apart and insulated from each other by the insulating layer. An orthographic projection of each individual one of the plurality of first electrodes on the base substrate at least partially overlap with an orthographic projection of the auxiliary electrode layer on the base substrate. Each of the plurality of first electrodes is electrically connected to a pixel circuit configured to drive light emission in a respective one of the plurality of subpixel areas.