- 专利标题: L-ornithine production in eukaryotic cells
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申请号: US15556737申请日: 2016-03-10
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公开(公告)号: US10544435B2公开(公告)日: 2020-01-28
- 发明人: Jiufu Qin , Anastasia Krivoruchko , Florian David , Bo Jiang , Jens Nielsen
- 申请人: BIOPETROLIA AB
- 申请人地址: IE Dublin
- 专利权人: CHRYSEA LIMITED
- 当前专利权人: CHRYSEA LIMITED
- 当前专利权人地址: IE Dublin
- 代理机构: Myers Bigel, P.A.
- 国际申请: PCT/SE2016/050193 WO 20160310
- 国际公布: WO2016/144247 WO 20160915
- 主分类号: C12P13/10
- IPC分类号: C12P13/10 ; C12N9/02 ; C12N9/10 ; C12N9/12 ; C12N9/78 ; C12N9/88 ; C12N13/00 ; C12N15/52 ; C12P13/00
摘要:
The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.
公开/授权文献
- US20180105850A1 L-ORNITHINE PRODUCTION IN EUKARYOTIC CELLS 公开/授权日:2018-04-19
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