The utility model discloses a micro - sampling device of fence formula, the device include culture dish (1), netted fence (2) and apron (4), culture dish (1) is rectangle household utensils form, and the bottom is level and smooth, netted fence (2) comprise 8 12 squares or 8 12 4 triangular prisms or 8 12 8 triangular prisms, and netted fence (2) are outer the same along the body of the container with culture dish (1) (3) inside dimension. Use the utility model discloses the problem that traditional separation method is consuming time, consume power, accuracy difference both can have been solved to the device, can provide novel sampling device for unicellular (or monospore) separation method again.