The utility model discloses a beta-stimulant seven-in-one colloidal gold detecting card. The beta-stimulant seven-in-one colloidal gold detecting card comprises a card housing and a detecting bottom plate which is arranged in the card housing, wherein a sample pad A, a conjugate pad, a sample pad B, a nitrocellulose membrane and a water absorbing pad are arranged on the detecting bottom plate in a fluid flow direction in sequence; a detecting line is arranged at one end, close to the sample pad B, of the nitrocellulose membrane; a quality control line is arranged at one end, close to the water absorbing pad, of the nitrocellulose membrane; brombuterol-carrier protein conjugate is coated on the detecting line; a goat anti-mouse IgG antibody is coated on the quality control line; an anti-clenbuterol mouse monoclonal antibody- colloidal gold conjugate is coated on the conjugate pad. According to the beta-stimulant seven-in-one colloidal gold detecting card disclosed by the utility model, the sample pad B is additionally arranged, so that the flow rate can be effectively controlled and the detection flexibility is improved. Moreover, the beta-stimulant seven-in-one colloidal gold detecting card can be used for high-flux online qualitative detection of residues of clenbuterol, salbutamol, tulobuterol, cimbuterol, mabuterol and terbutaline in swine urine, can be used for detecting a plurality of items and is capable of meeting the detecting needs.