The invention discloses a reagent kit for detecting an African swine fever virus nucleic acid test paper strip. The reagent kit contains a primer group for detecting African swine fever, wherein the nucleotide sequence of an upstream primer is shown as SEQID NO.1; the nucleotide sequence of a downstream primer is shown as SEQID NO.2; the mark of the upstream primer is Biotin, and the mark of the downstream primer is FITC. According to the reagent kit disclosed by the invention, virus DNA is directly obtained through tissue lysate, so that the step of nucleic acid extraction is not needed; through high-efficient nucleic acid amplification enzymes, the PCR reaction time is reduced; and the primer is marked, a nucleic acid agarose electrophoresis color development method is replaced by a nucleic acid test paper strip color development method. Through the adoption of the reagent kit disclosed by the invention, the method for efficiently and quickly inspecting African swine fever virus suitable for clinical usage is established; and compared with a conventional agarose gel electrophoresis method, the reagent kit does not need any instrument and equipment, is simple in operation, and hashigher sensitivity.