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发明公开
CN110218739A 一种监测pre-mRNA剪接过程报告基因影像探针及其构建方法 有权

一种监测pre-mRNA剪接过程报告基因影像探针及其构建方法
摘要：
本发明公开了一种监测pre-mRNA剪接过程报告基因影像探针及其构建方法。所述的探针包括载体pCDNA3.1(+)、基因片段BD、Rluc intron、AD，载体pG5 luc，所述的基因片段BD、Rluc intron、AD按照BD-Rluc intron-AD顺序与线性化的pcDNA3.1(+)载体重组。本发明的报告基因分子影像探针可以模拟活体pre-mRNA加工为成熟mRNA的过程，为模拟活体pre-mRNA加工过程的动态变化提供了一种实时、无创的监测工具，同时为作用于剪接体的药物研究提供了有效的筛选工具。
摘要（英）：

The invention discloses a reporter gene image probe for monitoring a pre-mRNA splicing process and an establishment method of the probe. The probe comprises a vector pCDNA3.1(+), a gene segment BD, anRluc intron, AD and a vector pG5 luc, the gene segment BD, the Rluc intron and the AD are in recombination with the linearized pcDNA3.1(+) vector according to a BD-Rluc intron-AD sequence. The reporter gene molecule image probe can simulate the process of processing in-vivo pre-nRNA into mature RNA, a real-time and noninvasive monitoring tool is provided for dynamic change of simulating the process of processing the in-vivo pre-nRNA, and an effective screening tool is provided for medicine research applied to a spliceosome.

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