The invention discloses a microbubble preparation for ultrasonic diagnosis and SDT (sonodynamic therapy) and a preparation method of the microbubble preparation. The microbubble preparation uses a rose-bengal long-chain compound and a surfactant as shell materials filled with gas, has the capabilities of ultrasonic imaging, ultrasonic located bursting and singlet oxygen production under ultrasonicstimulation, and can be used for tumor imaging and therapy. The drug loading capacity of microbubbles is improved effectively by drug synthesis and the microbubble preparation way, drug uptake of a tumor part is increased greatly by means of fixed-point bursting of the microbubbles by located ultrasound, and the tumor status is observed in real time through combination of ultrasonic imaging and therapy. The preparation can effectively increase diagnosis and treatment efficiency, is a high-efficiency novel drug loading system and has good clinical application prospects.