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发明公开
CN109234440A 一种用于检测芒果链格孢霉叶斑病病原菌的特异性引物及检测方法 无效 - 驳回

一种用于检测芒果链格孢霉叶斑病病原菌的特异性引物及检测方法
摘要：
本发明公开了一种用于检测芒果链格孢霉叶斑病病原菌的特异性引物及检测方法，主要提供了特异性引物(P1：5′-ACTTCTTGTTTCCTTGGTGGGT-3′和P2：5′-ACGCCCAACACCAAGCAA-3′)，采用该引物，可在芒果链格孢霉叶斑病菌基因组DNA或者疑似感染了芒果链格孢霉叶斑病病原菌的植物组织中特异性地扩增出280bp的特异扩增产物。所发明的特异性引物及其用法可被用于生产实践中芒果链格孢霉叶斑病菌的快速、灵敏、特异的检测，对芒果链格孢霉叶斑病病原菌的鉴定和防治具有十分重要的意义。
摘要（英）：

The invention discloses a specific primer for detecting Alternaria alternata leaf spot pathogen of mango and a detection method thereof, and mainly provides a specific primer (P1: 5 '- ACTTCTTGTTTCCTTGGTGGGT- 3 'and P2: 5'- ACGCCCAACACCAAGCAA- 3 ') of Alternaria alternata or plant tissue suspected to be infect with Alternaria alternata leaf spot pathogen of mango can be used to amplify a specificamplification product of 280bp. The invented specific primer and its application can be used for rapid, sensitive and specific detection of Alternaria mango leaf spot pathogen in production practice,and has very important significance for identification and control of Alternaria mango leaf spot pathogen.

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