The invention relates to a composition for preparing enzymes and an enzymes preparation method. The composition for preparing enzymes comprises fructus acanthopanacis senticosi, folium acanthopanacissenticosi, fructus crataegi, folium ginseng, mulberry, fructus schisandrae; the enzymes preparation method includes preparing of materials, sterilizing, fermenting, filtering and standing, concentrating and drying and the like; the prepared enzymes have functions of nourishing kidney, building the body, tonifying blood and qi, invigorating spleen and stomach, promoting digestion and removing foodretention and the like. The enzymes preparation method has the advantages of simple selection of materials, complete and comprehensive processing and low waste of resources; the powdered product prepared has small size and light weight and is convenient to carry with and convenient for users to make beverage for drinking; the enzyme beverage has pure taste, is environmental friendly and low in side effect, has good conditioning and health care effect to the human body after long-term application, is suitable for different groups of people, and has excellent technical effect.