The invention belongs to the field of medicines, and particularly relates to a pyrimidine phenylurea anti-tumor compound and a preparation method and application thereof. A structure general formula of the pyrimidine phenylurea anti-tumor compound is specifically as shown in the description. The compound has a certain inhibitory activity to a human lung cancer H460 cell, a cervical cancer Hela cell, and a human ovarian cancer OVCAR3 cell. In a synthetic process, the synthetic procedures are simplified, and the possibility is provided for the future industrial production.