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发明授权
CN108411005B MTHFR基因片段作为母猪肢蹄骨密度相关的SNP分子标记 有权

MTHFR基因片段作为母猪肢蹄骨密度相关的SNP分子标记
摘要：
本发明属于动物分子标记筛选技术领域，具体涉及MTHFR基因片段作为母猪肢蹄骨密度相关的SNP分子标记。该分子标记克隆自登陆号为M1GA0008657的MTHFR基因片段，通过基因芯片技术对该基因进行分型筛选得到一种与母猪肢蹄骨密度相关的分子标记，该标记的核苷酸序列如SEQ ID NO：1所示，在该序列的第51位的碱基处存在一个A/C的等位基因突变，当SEQ ID NO：1上的第51位的核苷酸为A时，则母猪的肢蹄具有更高的骨密度。公开了筛选与母猪肢蹄骨密度相关分子标记的方法及其关联分析方法的应用。本发明为猪肢蹄结实度性状尤其是骨密度的标记辅助选择提供了一个新的SNP分子标记。
摘要（英）：

The invention belongs to the technical field of animal molecular marking and particularly relates to MTHFR (methylenetetrahydrofolate reductase) gene fragment as an SNP (single-nucleotide polymorphism) molecular marker associated with sow foot-leg bone mineral density. The molecular marker is cloned from the MTHFR gene fragment having the register number of M1GA0008657; the gene fragment is subjected to typing screening via a gene chip technology so as to obtain the molecular marker associated with sow foot-leg bone mineral density; a nucleotide sequence of the molecular marker is shown as inSEQ ID NO: 1; base 51 of the nucleotide sequence has an A/C allele mutation; when nucleotide 51 in the SEQ ID NO: 1 is A, sow leg-foot bone has higher bone mineral density. Disclosed a method of screening molecular markers associated with sow pig-leg bone mineral density, as well as application of association analytical method. The novel SNP molecular marker is provided for marking-assisted selection of pig leg-foot compactness traits, particularly bone mineral density.

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