The invention discloses a hand die conveying and assembling device for glove production. The hand die conveying and assembling device comprises a bearing unit, a power unit, movable assembling units,a hand die unit and a tight holding unit. The bearing unit comprises an extension piece, a vertical jacking piece and a transverse support piece, wherein the extension piece is connected with one endof the transverse support piece, and the transverse support piece and the vertical jacking piece are vertically arranged. The power unit surrounds the periphery of the bearing unit and comprises a first drive piece and a conveying piece, and the conveying piece is connected with the first drive piece. The movable assembling units are arranged at the two ends of the vertical jacking piece. The handdie unit is connected with a chain of the conveying piece. The tight holding unit is arranged on a gear shaft of the conveying piece, and the conveying piece further comprises a transmission gear andthe chain. According to the hand die conveying and assembling device, the tight holding and braking effects in the emergency situation can be effectively achieved in a mechanical transmission and hydraulic transmission combining manner, and the hand die conveying and assembling device is convenient to operate and has an excellent braking effect; and the one-to-many tight holding function can be achieved, and structures at multiple positions can be braked at the same time.