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发明授权
CN108359655B 一种热稳定性高的脂肪酶突变体TDL-mut及其编码基因 有权

一种热稳定性高的脂肪酶突变体TDL-mut及其编码基因
摘要：
本发明属于蛋白质工程领域，涉及一种热稳定性高的脂肪酶突变体TDL‑mut及其编码基因。本发明所述的脂肪酶突变体TDL‑mut的突变位点为：突变位点为F12C，R42S，G43E，S44N，I45V，I98L，N99A，L100A，K103T，G104E，T231N和K237C，进而获得一种热稳定性提高的1，3位置特异性脂肪酶突变体TDL‑mut。该突变体TDL‑mut在80℃水浴5min后的酶活保留率为70％，而脂肪酶TDL在80℃水浴5min后的酶活保留率仅为18％。相对于脂肪酶TDL，突变体TDL‑mut耐热性的提高幅度为288％。因此，本发明的获得的脂肪酶突变体TDL‑mut具有良好的热稳定性，为其产业化应用奠定基础。
摘要（英）：

The invention belongs to the field of protein engineering, and relates to a lipase mutant TDL-mut having high thermal stability and a coding gene of the lipase mutant TDL-mut. Mutation sites of the lipase mutant TDL-mut are as follows: F12C, R42S, G43E, S44N, I45V, I98L, N99A, L100A, K103T, G104E, T231N and K237C; therefore, the 1,3 site-specific lipase mutant TDL-mut that the thermal stability isimproved is obtained. An enzymatic activity retention rate of the mutant TDL-mut, which undergoes a water bath at 80 DEG C for 5min, is kept at 70%, while an enzymatic activity retention rate of lipase TDL, which undergoes a water bath at 80 DEG C for 5min, is merely kept at 18%. In comparison with the lipase TDL, heat resistance of the mutant TDL-mut is increased by an amplitude of 288%. Therefore, the lipase mutant TDL-mut, obtained from the invention, is good in thermal stability; and a foundation is laid for industrial application.

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